2^nd International Conference on Nutraceuticals and Nutrition Supplements July 18-19, 2016 Bangkok, Thailand

Session Introduction

Seung-Kwon Myung

National Cancer Center, South Korea

Title: Efficacy Of Vitamin And Antioxidant Supplements In Prevention Of Bladder Cancer: Meta Analysis Of Randomized Controlled Trials

Biography:

Seung-Kwon Myung has received his MD from Seoul National University College of Medicine and has completed his PhD in Family Medicine from the same university. He is an Associate Professor of Graduate School of Cancer Science and Policy, National Cancer Center, Korea. He has published 63 papers in reputed medical journals and served as an Editor-In-Chief of the Korean Journal of Family Medicine. His research specialty is meta-analysis and his research areas are cancer prevention and food, specifically the efficacy of vitamin supplements and functional foods on health.

Abstract:

This meta-analysis aimed to investigate the efficacy of vitamin and antioxidant supplements in the prevention of bladder cancer as reported by randomized controlled trials (RCTs). We searched PubMed, EMBASE and the Cochrane Library in April 2015. Two of the authors independently reviewed and selected eligible RCTs, based on pre-determined selection criteria. Out of 180 articles searched from three databases and relevant bibliographies, 14 RCTs were included in the final analysis. In a fixed effect meta-analysis, there was no efficacy of vitamin and antioxidant supplements in the prevention of bladder cancer (relative risk (RR), 1.03; 95% confidence interval (CI), 0.91-1.16; I2=43.4%). Overall, there was no preventive effect of these supplements in the subgroup meta-analyses by various factors. Among the subgroup analyses by type of supplements, beta-carotene supplementation alone was marginally increased the risk of bladder cancer (RR, 1.44; 95% CI, 1.00-2.09; I2=0.0%; n=3). The current meta-analysis of RCTs found that there was no clinical evidence to support the efficacy of vitamin and antioxidant supplements in the prevention of bladder cancer.

Session Introduction

Jiankang Liu

Professor & Dean, Xi’an Jiaotong University, China

Title: Targeting mitochondria to prevent age-associated diseases with nutraceuticals: Hydroxytyrosol is a rising star after resveratrol

Time : 10:05-10:40

Biography:

Jiankang Liu has received his BS from Xi’an Jiaotong Unviersity in 1982 and PhD of Medical Science from Okayama University School of Medicine in 1994. He has completed Post doctorate training in Dr. Bruce Ames Laboratory at University of California, Berkeley and worked as a Faculty at University of California at Berkeley, Children Hospital Oakland Research Institute, University of California at Irvine, University of Kentucky College of Medicine and Shanghai Institute for Nutritional Science, Chinese Academy of Sciences. Currently, he is a Professor and the Dean of Xi’an Jiaotong University School of Life Science and Technology and an Adjunct Professor of the School of Pharmacy, University of Southern California. His research interests include molecular and cellular mechanisms of aging, stress and age-/stress associated degenerative diseases with a focus on nutritional regulation of mitochondrial metabolism. He has published more 150 papers in international journals with more than 4000 times citations (H-index 37) and was elected as one of the “2014 Most Cited Chinese Researchers” in the area of Biochemistry, Genetics and Molecular Biology by Elsevier

Abstract:

Due to the limitation and ineffectiveness of antioxidants in extending life span and preventing age-related diseases, scientists have being tried to find new solutions beyond antioxidants for delaying aging and preventing age-related diseases. The French paradox-derived resveratrol has become a star of nutraceutical because of the discovery of its effect on Sirtuins regulation by Sinclare’s lab at Harvard on. Nowadays, resveratrol has been widely studied on retarding aging and preventing/ameliorating numerous disorders in cellular and animal models as well as clinical studies. We have recently investigated a number of naturally occurring nutraceuticals and found that hydroxytyrosol (HT) has a unique and potent effect on regulating mitochondrial metabolism (biogenesis and degradation). HT is abundant in olives and virgin olive oil, which are widely used in Mediterranean diets. Epidemiologic studies have demonstrated that the people of the Mediterranean basin enjoy a healthy lifestyle with decreased incidence of heart disease, prostate and colon cancer and rheumatoid arthritis. One of the possible reasons is that Mediterranean people have a high intake of HT from olive and olive oil. The beneficial effects of HT have been mainly attributed to its antioxidant effect. However, we have demonstrated that HT is an effective mitochondria-targeting nutraceutical in various cellular and animal models, including age-associated macular degeneration, metabolic disorders, cancer, osteoporosis and muscle atrophy. Because mitochondrial dysfunction has been implicated in aging and various diseases the potent effects of HT on regulating mitochondrial metabolism suggest that HT is a rising star of nutraceutical after resveratrol and will be widely used for delaying aging and preventing/ameliorating age-associated diseases

Chandan Prasad

Texas Woman’s University, USA

Title: Pharmacobiology of cyclic dipeptides: Neuroceuticals to nutraceuticals

Time : 09:30-10:05

Biography:

Chandan Prasad has completed his PhD in Microbiology/Biochemistry at Louisiana State University in 1970. He has served LSU Health Sciences Center as Professor (Medicine and Neuroscience), Director of Obesity Research Program and Vice Chairman in the Department of Medicine. He has joined Texas Woman’s University as Professor and Chair of the Department of Nutrition and served till 2012. He has also served TWU as Assistant Provost for Research Promotion. Currently, he is a Professor of Nutrition and Food Sciences at the Texas Woman's University and Professor (Emeritus) of Medicine (Endocrinology) at the LSU Health Sciences Center, New Orleans, LA. The current focus of his research is on inflammation and the role of dietary supplements in obesity, diabetes and heart diseases. He has authored more than 200 papers in the area off appetite regulation, obesity, and nutrition. He holds four US and international patents for treatment of obesity and alcoholism. He is the Founding Editor of Nutritional Neuroscience, Series Editor for Nutrition, Brain and Behavior, Editor-in-Chief of Current Topics in Nutraceutical Research and Member of the Board of the International Society of Nutrigenetics/Nutrigenomics.

Abstract:

Cyclic dipeptides consist of a broad array of natural as well as products resulting from food processing that exhibit useful biological properties. Diketopiperazines are the smallest cyclic peptides that have been shown to exist in bacteria, fungi and higher organisms as well as processed food. These diketopiperazines have been shown to exhibit a variety of biological/pharmacologic activities including, antitumor, antiviral, antifungal, antibacterial, antiprion, antihyperglycemic, antineurotoxic and many others. The answer to the diversity of the biological/pharmacologic activities associated with these diketopiperazines perhaps lies in their structural differences and specificity in the mechanism of action. In this presentation, using cyclo (His-Pro) as an example, I will present a historical overview of this rapidly expanding field that will focus on mechanism of action when possible. Lastly, there will be a brief comment on the possible use of some of these diketopiperazines as nutraceuticals/pharmaceuticals.

Session Introduction

Norihisa Kato

Hiroshima University, Japan

Title: The role of vitamin B6 in health and diseases

Biography:

Norihisa Kato has completed his PhD (1980) from Nagoya University, Japan. Currently, he is a professor in Laboratory of Molecular Nutrition, Graduate School of Biosphere Science, Hiroshima University. His research interests are in elucidation of anti-disease food factors and the molecular mechanisms. He is a expert editor of Journal of Nutritional Science and Vitaminology, and has published more than 200 papers. He was given the Award for Excellence in Research Japan Society of Nutrition and Food Science (2012).

Abstract:

Although vitamin B6 is widely distributed in various foods, there is evidence that many adults are not obtaining adequate amounts of this nutrient from their diets, implying that the nutritional importance of vitamin B6 is under-recognized. In 2001, our group provided the first evidence for the anti-colon tumor effect of dietary supplemental vitamin B6 in mice received azoxymethane. Since then, a number of epidemiological studies have suggested an inverse association between vitamin B6 status and the risk of cancers. Furthermore, there is accumulating epidemiological evidence suggesting anti-cardiovascular and anti-inflammation effects of vitamin B6. Studies in our laboratory have indicated anti-inflammatory, anti-proliferative, anti-angiogenesis, and anti-oxidant effects of vitamin B6 in animals and cell culture studies. Recent our studies have revealed novel anti-inflammatory activities of vitamin B6, suggesting potential utility of vitamin B6 in preventing NLRP3 inflammasome-driven inflammatory diseases. We further found the elevation of carnosine and anserine (anti-inhibitory, anti-oxidant and ergogenic dipeptides) in heart and skeletal muscles of rats by dietary supplemental vitamin B6. These findings explain why vitamin B6 has important role in the anti-disease functions and health. In this lecture, I will present recent progress in the studies on these functions of vitamin B6.

Nicolas Wiernsperger

Lyon University, France

Title: Mechanisms common to chronic diseases may be the best targets for nutraceutical approaches

Biography:

Nicolas Wiernsperger has completed his PhD from Basle University, Switzerland. He was a Researcher at Novartis (Basle, Switzerland) and later a Research Director at Lipha/Merck KgA in Lyon, where he was chairing the international pharmacological development as well as a public/private research unit at Lyon University. He is an internationally recognized specialist in pathophysiology and pharmacology of microcirculation, metabolic syndrome and diabetes. He has published more than 140 papers in reputed journals, Editor of 2 books and has been serving as an Editorial Board Member.

Abstract:

Chronic degenerative diseases characterizing aging human beings share some common key mechanistic defects which, according probably to epigenetic and environmental factors, direct progressions towards the one or other pathology. One such key step is cellular resistance to insulin. Recent research has identified insulin resistance as a defect found in metabolic and vascular processes going far beyond the “classical” view of insulin’s biological effects on glucose metabolism. As a ubiquitous phenomenon showing an extremely high prevalence in the population, insulin resistance is now increasingly seen as an early occurring cellular disturbance affecting most various diseases. These observations then point to the likelihood of common origins of insulin resistance. Although multiple defects in insulin cellular action have been unraveled over the two last decades, it appears that one common denominator is chronic low grade inflammation. While the latter it may have different causes, mechanistic analysis of insulin resistance microvascular and metabolic disturbances shows that the chronic, even moderate augmentation of inflammatory substances (largely cytokines) is key to its genesis. The author will give a complete overview of these commonalities, which may explain the long term development of the main diseases affecting worldwide aging population. Since these defects are both mild and very lasting, they may be best suited as targets for nutraceutic therapy.

KP Mohana Kumar

Director & Professor, Mahatma Gandhi University, Kerala, Inida

Title: Synergistic actions of nutraceuticals with drugs: Neuro-nutraceuticals & CNS drugs

Time : 11:00-11:35

Biography:

Mohanakumar KP is the Director of Inter University Centre for Biomedical Research & Super Specialty Hospital, a Govt of Kerala autonomous institution with Mahatma Gandhi University, Kottayam, Kerala, India. He graduated from Saurashtra University with a Master’s degree in Chemical Neuroanatomy and PhD in Neurochemistry. He was Chief Scientist at CSIR-Indian Institute of Chemical Biology, Kolkata, where he has established a laboratory on neurodegenerative diseases, with special reference to Parkinson's disease, in relation to pathogenesis, progression of the disease and its treatment. His cybrids laboratory facility is recognized for his interest in mitochondrial involvement in neurodegenerative diseases. He has more than 125 peer reviewed publications, and guided 30 PhD students in the field of Neuropharmacology, and more than a 100 postgraduates in Medicine, Pharmacology, Biotechnology, Biochemistry and other biomedical discipline. A fellow of the National Academy of Sciences, India; West Bengal Academy of Science & Technology and Indian Academy of Neurosciences, he received National Bioscientist Award from DBT, Govt. of India and ICMR, Govt. of India Young Scientist Prize. He is a Professor of Biological Sciences of AcSIR, India and served as Professor of TWAS-UNESCO and IISER, Thiruvananthapuram. He serves as editorial member of J Neurochem, Neurochem Int, Neurochem Res, Neurosci Med, Anat Cell Biol, etc.

Abstract:

Many molecules contained in our daily intake of food act as nutraceuticals, and its concentration, time of intake and duration for which it is available in the body are critical to influence human health. Diet-rich in nutraceuticals or supplementation of identified nutraceuticals in the diet along with yoga, exercise, increased outdoor activities and maintenance of a reasonably balanced body weight will maximise a healthy mind and body with reduced disease risk. It is amazing how health prospects are bettered by simply changing food habits, increasing availability of nutraceuticals in the diet either with nutraceutical supplements or introducing nutraceutical rich diet or increasing fermented foods. A healthy mind is integral to a healthy body, and considering genetic predisposition of an individual, nutraceuticals will act as substrates of biochemical reaction, co-factors or inhibitors of enzymes, absorbents that binds to undesirable molecules in the GI tract, scavengers of toxic molecules and free radicals, enhance of absorption of micronutrients, help to balance micro-biome of the gut, and maintain a healthy brain-gut axis, resulting in variable reversal of traumatic, inflammatory, toxic, immune and vascular damages to nervous tissue. However, chronic neurological diseases, which results from many causes and risk factors such as genetic predisposition, advanced unhealthy lifestyle, exposure to toxins/toxic chemicals, infections, immuno-deficiencies, adverse environment causing epigenetic mechanisms going haywire, and co-morbidities from diabetes, and cardiovascular complications cannot be cured or addressed successfully with diets, nutritive supplements, or herbals or phytochemicals, but should be treated with prescription drugs. The nutraceuticals while interacting with prescription drugs, do they synergize with modern drug molecules to provide better health prospects or do they complicate the drug effects to worsen the side effects situations over long-term is a major issue? This is important since more than 10% of all apparent decline in neurological disorders, such as cognition, sensory and motor functions, GI track immobility, etc are resulting from iatrogenic effects due to the overuse of OTC or prescription drugs. Neuro-nutraceuticals are the new horizons that might just help to address this issue. Evidences are getting accumulated to support the notion that even the primary drug effects could be synergized by neuro-nutraceuticals, solving the issues of tolerance and toxicity due to higher drug doses. Neuro-nutraceuticals might work through specific messenger molecules that cross over from the regulation of GI tract and immune functions to neuronal function, and vice versa. It has become a way for neurologists to treat CNS diseases and neuropsychaitric conditions (eg. hepatic encephalopathy, inflammatory disease, obsessive compulsice disorders,attention deficit hyperactive disorders, depression, several neurodegenerative diseases, etc.) with 'functional foods', and this has turned its focus on botanicals and/or nutritional phytochemicals, essential fatty acids or its natural sources, biologicals such as phosphatidylserine, acetyl-l-carnitine, vinpocetine, vitamins, glutathione, α-lipoic acid, n-acetyl-cysteine, bioflavonoids, flavone glycosides, phytooestrogens, coenzyme Q10, s-adenosyl methionine, etc. Not but the least, habit of tea or coffee or wine drinking is also shown to synergize with neurological drugs to provide better therapeutic outcome, and in significant cases it has helped to reduce the dosage of prescription drugs.

Session Introduction

Chandan Prasad

Texas Woman’s University, USA

Title: Agri-byproducts, an unexplored resource for health promotion

Time : 11:35-12:00

Biography:

Chandan Prasad graduated from Louisiana State University in 1970 with a Ph.D. in microbiology/biochemistry. After 8 years at the NIH in Bethesda, Maryland as Fogerty fellow and senior staff fellow, he returned to New Orleans to join the faculty of LSU School of Medicine. He served LSU Health Sciences Center as professor (medicine and neuroscience), Director of Obesity Research Program, and vice chairman (research) in the Department of Medicine until 2006. He was the first chair of the laboratory of Nutrition and Brain at the Pennington Biomedical Research Center from 1991-1993 under directorship of Prof. George Bray MD. He joined Texas Woman’s University as Professor & Chair of the Department of Nutrition and served in this capacity until 2012. He also served TWU as Assistant Provost for Research Promotion from 2012 to 2014. Currently, he is Professor of Nutrition and Food Sciences at the Texas Woman's University and Professor (Emeritus) of Medicine (Endocrinology) at the LSU Health Sciences Center- New Orleans, LA. The current focus of Dr. Prasad’s research is on inflammation and the role of dietary supplements in obesity, diabetes, and heart diseases. He has authored more than 200 papers in the area off appetite regulation, obesity, and nutrition. He holds four U.S. and international patents for treatment of obesity and alcoholism. He is Founding Editor of Nutritional Neuroscience, series editor for Nutrition, Brain, and Behavior, Editor-in-Chief of Current Topics in Nutraceutical Research and Member of the Board of the International Society of Nutrigenetics/Nutrigenomics .

Abstract:

Agri-byproducts result from agricultural activities such as cultivation of rice, wheat, barley, corn, sugarcane, banana and other crops as well as food processing and manufacturing. These byproducts are rich source of arabinogalactans, cellulose, hemicellulose and lignin and a variety of other compounds. While some of these byproducts have used for production of fibers, ethanol and chemicals such as furfural, the most go as animal feed, compost or waste. Significant progress in analytical/processing chemistry and biotechnology methods in the last decades has opened the possibility of developing new uses of agri-byproducts commonly referred to as agri-wastes. The cellulosic byproducts are well suited to be converted into high value nutraceuticals (beta-Glucans, Resistant Starch, Glucomannan, Chitosans, Arabinoxylans) capable of reducing inflammation, enhancing immune response and arresting cell growth of cancer cells. This presentation will summarize existing data and suggest future directions on identification, characterization, derivatization, biologic testing and function of agricultural byproducts as possible nutraceuticals for human use.

Zhao-Jun Wei

Hefei University of Technology, China

Title: The nutraceutical properties of polysaccharides sequential extracted from peony seed dreg

Biography:

Zhaojun Wei has received his BS from Anhui Agricultural University (1993), MS from Chinese Academy of Agricultural Sciences (2001) and PhD from University of Science and Technology of China (2005). Currently, he is the distinguished Professor and Deputy Dean of School of Biotechnology and Food Engineering at Hefei University of Technology (HFUT). He also serves as the Chairman of the Agricultural and forestry specialty food processing industry technological innovation strategic alliance of Anhui province, Chairman of the Hefei Society for Nutrition, and a Member of the Chinese Society of Biochemistry and Molecular of Agriculture. His research focuses on the processing and function of nutraceuticals from the byproducts of Agriculture and forestry, e.g., DNJ from mulberry, Pectin from okra, polysaccharides from peony seed dreg and others.

Abstract:

Peony seed dreg, a byproduct of oil processing, is rich in many nutrients. However, this is always used as animal fodder and fertilizer. My laboratory has explored the possible use of nutrient-rich peony seed dreg as a functional food. To this end, Peony seed dreg polysaccharides (PSDP) were sequentially extracted using hot buffer (HBSS), chelating agent (CHSS) and dilute alkaline (DASS) followed by concentrated alkaline (CASS). The nutraceutical properties of the various polysaccharides fractions were investigated. The four PSDPs possessed simple composition and showed the characteristic absorptions for polysaccharides at 3600-3200 cm-1 and 3000-2800 cm-1. The functional group of four PSDPs displayed some slight differences. The results of melting point and enthalpy of four samples confirmed a good thermal stability. The emulsifying activity and emulsifying stability of four PSDPs exhibited dose-dependent response, HBSS with the highest emulsifying activity and CHSS with the longest emulsifying stability. Comparison among the antioxidant activities of four PSDPs, CASS showed highest abilities in the DPPH radical scavenging activity, reducing power and ABTS radical scavenging activity, HBSS exhibited the highest hydroxyl radical scavenging activity and CHSS displayed the higher chelating ability on ferrous ions than others. At a liquid-solid ratio less than 2.5% w/w in aqueous solution, the polysaccharides extracted from peony seed presented non-Newtonian shear thinning behavior and could be well described by the Cross model. The peony seed polysaccharides showed predominantly viscous responses (G’

Lin shaoling

Fujian Agriculture and Forestry University, China

Title: Sensory and nutritional properties of chinese olive pumace based high fiber biscuit

Biography:

Shaoling Lin has completed her PhD from The Chinese University of Hong Kong.

Abstract:

Consumer interest in healthy eating is shifting towards the potential health benefits of specific foods and food ingredients. Unconventional ingredients combined with usual bakery flour yields better acceptable, healthy and nutritious bakery products. The present study aims to evaluate the sensory and nutritional properties of Chinese olive pumace based high fiber biscuit. The high fiber biscuit was formulated using Chinese olive pumace powder (15%), wheat (100 g), sugar powder (33%) and spices after repeated trials and the recipe was standardized. When subjected to sensory evaluation, the newly formulated high fiber biscuit derived a score of 4.5±0.51, out of a maximum score of 5, for its overall acceptability, indicating the biscuit was well accepted. The results of the nutritional quality and cost of the newly formulated high fiber biscuit showed that it contains the 10.20 g fiber, 1454 Kcal calories and 18.30 g fat in 100 g biscuit. Furthermore, the glycemic index (GI) results indicated that GI of olive high fiber biscuits was 63.68 which was significantly lower than the control one with the GI value of 76.66. Besides, the physical and chemical indicators as well as the microbiological indicators were also up to the Chinese national standards (GB/T20980-2007 and GB7100-2003). Hence, the study proved that the newly formulated olive pumace powder based biscuit is well acceptable based on its sensory properties and fiber rich nutritional quality.

Ying Ma

Harbin Institute of Technology, China

Title: Binding characteristics of curcumin to β-Lactoglobulin

Time : 14:00-14:25

Biography:

Professor Ying Ma has completed her PhD from Northeast Agricultural University and postdoctoral studies from Chinese Agricultural University. Now Dr. Ma, as a professor and doctor student’s supervisor, works in institute of food science and engineering of Harbin Institute Technology. She also is associate editor of an international journal《Dairy Science and Technology》(formerly le lait). Her research area mainly focuses on the food chemistry, food safety，the modifications of starch and plant protein, and bioprocess of agricultural food. As a coordinator, she jointed several national and local research projects. More than 248 peer- reviewed research papers were published in primary journals. She owned 21 authorized patents and 22 edited Chinese books and 5 peer-reviewed English books.chapters.

Abstract:

Curcumin (CCM) is a phenolic compound and has been shown to exhibit many bioactivities, but it is extremely limited in its application due to its insolubility in water. β-Lactoglobulin (β-Lg) is a major whey protein and has an ability to bind hydrophobic molecules because of its hydrophobic core. In this experiments, the binding of CCM to β-Lg was investigated. The results showed that binding CCM to β-Lg leads to a partial change in protein structure. CCM was bound respectively to two different sites of protein at pH 6.0 and pH 7.0 via hydrophobic interaction. CCM-β-Lg complex was formed by one molecule of protein combining with one molecule of CCM. The solubility of CCM was increased by 15954-fold through the complex. The β-Lg -CCM complex and CCM in complex had a good stability in the pH range of 2 to 8. The results of antioxidant experiments showed that the ABTS and hydroxyl radical scavenging capacity of CCM was reduced by binding with β- Lg, but the total reducing ability of CCM was improved.

Jiankang Liu

Xi'an Jiaotong University, China

Title: Targeting mitochondria for preventing and treating metabolic syndrome with natural compounds from food and nutrition

Time : 14:25-14:50

Biography:

Jiankang Liu has received his BS from Xi’an Jiaotong University and PhD of Medical Science from Okayama University School of Medicine, Japan. He has completed Post doctorate training in Dr. Bruce Ames Laboratory at University of California, Berkeley and worked as a Faculty at University of California at Berkeley, Children Hospital Oakland Research Institute, University of California at Irvine, University of Kentucky College of Medicine and Shanghai Institute for Nutritional Science, Chinese Academy of Sciences. Currently, he is a Professor and the Dean of Xi’an Jiaotong University School of Life Science and Technology and an Adjunct Professor of the School of Pharmacy, University of Southern California. His research interests include molecular and cellular mechanisms of aging, stress and age/stress associated degenerative diseases with a focus on nutritional regulation of mitochondrial metabolism.

Abstract:

Insulin resistance is an important feature of metabolic syndrome such as type-2 diabetes, obesity and cardiovascular disease. The underlying mechanisms of insulin resistance are still unclear. Mitochondrial dysfunction, including disorders of mitochondrial biogenesis and mitophagy and imbalance of redox system has been suggested to be involved in insulin resistance. Increasing evidence suggests that targeting mitochondria to protect mitochondrial function could prevent and ameliorate various diseases associated with mitochondrial dysfunction forming a unique field, i.e., mitochondrial medicine. In this presentation, I have summarized our recent studies with nutrients to target mitochondria by stimulating mitochondrial homeostasis to improve mitochondrial function and regulate redox balance for preventing and ameliorating metabolic syndrome. We have focused on natural compounds from food and nutrition including olive, bitter gourd and pomegranate to regulate mitochondrial biogenesis and degradation in cellular systems and in animal models. The in vitro and in vivo studies on the effects and mechanisms of mitochondrial targeting nutrients or their combinations may help us to understand the importance and mechanisms of mitochondrial remodeling and to develop mitochondria-targeting agents for preventing and treating obesity, diabetes and cardiovascular disease.

Rolando L. Maddela

Senior Director of Clinical Research and Scientific Affairs, Mannatech Incorporated, USA

Title: Arabinogalactan and other polysaccharides in health

Time : 14:50-15:15

Biography:

Dr. Maddela is the Senior Director of Clinical Research and Scientific Affairs of Mannatech, Incorporated located in Coppell, United States. He oversees the administration of product clinical trials and the product Safety Monitoring Program. Before joining Mannatech in 2005, he was for 20 years involved with several major public health programs in the Asia Pacific Region working with the academe, government, non-profit and international organizations. He earned a Bachelor of Science in Public Health from the College of Public Health, University of the Philippines, Manila and his MD degree from the University of the East Ramon Magsaysay Memorial Medical Center (UERMMMC), Philippines. In 1990 he obtained his Master of Public Health (MPH) degree with Distinction from the Braun School of Public Health and Community Medicine at the Hebrew University in Jerusalem, Israel. While in the United States, Dr. Maddela has completed several coursework on Public Health, Clinical Research, Data and Project Management, and Leadership in other US academic and professional institutions. He is a Certified Clinical Research Professional (CCRP) and has directed or monitored to successful completion several preclinical and clinical studies conducted in collaboration with academic or other research organizations.

Abstract:

Exogenous polysaccharides play many roles in various biological processes including health promotion by enhancing immune function. For example polysaccharide products derived from the larch tree (Larix spp.), brown algae (Undaria pinnatifida) and Aloe vera (Aloe barbadensis Mill) have been studied alone or in combination for immune function enhancement. Since these polysaccharides are generally considered innocuous, health care providers are very interested in their efficacy and safety following oral intake. The recent clinical studies, although still few compared to in vitro and animal studies, have so far demonstrated that oral intake of arabinogalactans, fucoidans and mannans can modulate immune response in humans. Furthermore, these polysaccharides are well tolerated. Although there is increasing awareness of oral polysaccharides’ multiple roles in health including their immunomodulatory actions, further research is warranted to help understand the spectrum of other health effects and to help explain the mechanisms underlying some of the observed health benefits.

Chandan Prasad

Texas Woman's University, USA

Title: Management of hyperuricemia in prepubertal children: Is there a role for functional fibers?

Time : 15:15-15:40

Biography:

Chandan Prasad graduated from Louisiana State University in 1970 with a Ph.D. in microbiology/biochemistry. After 8 years at the NIH in Bethesda, Maryland as Fogerty fellow and senior staff fellow, he returned to New Orleans to join the faculty of LSU School of Medicine. He served LSU Health Sciences Center as professor (medicine and neuroscience), Director of Obesity Research Program, and vice chairman (research) in the Department of Medicine until 2006. He was the first chair of the laboratory of Nutrition and Brain at the Pennington Biomedical Research Center from 1991-1993 under directorship of Prof. George Bray MD. He joined Texas Woman’s University as Professor & Chair of the Department of Nutrition and served in this capacity until 2012. He also served TWU as Assistant Provost for Research Promotion from 2012 to 2014. Currently, he is Professor of Nutrition and Food Sciences at the Texas Woman's University and Professor (Emeritus) of Medicine (Endocrinology) at the LSU Health Sciences Center- New Orleans, LA. The current focus of Dr. Prasad’s research is on inflammation and the role of dietary supplements in obesity, diabetes, and heart diseases. He has authored more than 200 papers in the area off appetite regulation, obesity, and nutrition. He holds four U.S. and international patents for treatment of obesity and alcoholism. He is Founding Editor of Nutritional Neuroscience, series editor for Nutrition, Brain, and Behavior, Editor-in-Chief of Current Topics in Nutraceutical Research and Member of the Board of the International Society of Nutrigenetics/Nutrigenomics

Abstract:

Although uric acid was first identified some two centuries ago, newer pathophysiologic aspects of hyperuricemia continue to emerge. For years, hyperuricemia has been thought to be exclusively associated with Gout, but it has now been associated with a number of metabolic and hemodynamic abnormalities in adults. More recently, we have examined association between hyperuricemia and components of metabolic syndrome (dyslipidemia, obesity, insulin resistance, and elevated blood pressure) in prepubertal obese school children from underserved population of Nuevo Leon, Mexico. To our surprise, we observed that compared to normal weight children, children in the obese group had significantly greater waist circumference, higher systolic and diastolic blood pressure, elevated fasting insulin and insulin resistance (IR), higher triglycerides, serum uric acid (sUA) and lower HDL-cholesterol. Among the obese children, 33% had characteristics of MetS and 57% of these children had sUA between 5.1-7.1 mg/dl. sUA was positively associated with IR and dyslipidemia. We cannot be certain at this time whether hyperuricemia is the cause or effect in the sequelae of the disease process. However, management of hyperuricemia in young children will be problematic since pharmacologic agents used to treat the condition are largely for adults with no experience with children and plethora of side effects. Therefore, I would like to summarize available data exploring the possibility of managing hyperuricemia in children with functional fibers.

Norihisa Kato

Hiroshima University, Japan

Title: Aspergillus derived protease as a novel bifidogenic factor

Biography:

Norihisa Kato has completed his PhD (1980) from Nagoya University, Japan. Currently, he is a professor in Laboratory of Molecular Nutrition, Graduate School of Biosphere Science, Hiroshima University. His research interests are in elucidation of anti-disease food factors and the molecular mechanisms. He is a expert editor of Journal of Nutritional Science and Vitaminology, and has published more than 200 papers. He was given the Award for Excellence in Research Japan Society of Nutrition and Food Science (2012).

Abstract:

Traditionally, Aspergillus species have been widely used for the production of a variety of fermented Japanese foods including, miso, soy sauce, sake, and sake lees. However, there is limited study on the application of Aspergillus species for the production of functional foods beneficial for health. In a recent animal study, we observed a marked elevation in the colon Bifidobacterium and organic acids in rats fed Aspergillus-fermented burdock compared to burdock powder (Okazaki et al. Biosci Biotechnol Biochem 77: 53, 2013). Similar observations were made in rats fed with water-soluble fraction from the fermented burdock and other Aspergillus-fermented foods such as malted rice and multi-grain malt. Since the water-soluble fraction from the fermented burdock contained substantial amount of extracellular proteases derived from Aspergillus, we postulated that bifidogenic effects might be associated with Aspergillus-derived proteases. To test hypothesis, rats were fed a diet containing 0.1% protease A ‘Amano’ SD (Aspergillus oryza，Amano Enzyme Inc.) (Amano protease) and number of Bifidobacterium in colon was measured. The results showed a marked elevation in colon Bifidobacterium population (Yang et al. Biomed Rep 3: 715, 2015). Since the Amano protease preparation is a mixture of several proteases, we set out to identify the active protease responsible for the bifidogenic effect. Our results show an acid protease derived from Aspergillus oryzae to exhibit a potent bifidogenic effect that was lost when protease was inactivated. A dietary addition of 0.0384% acid protease was comparable to 5 to 10% of prebiotics (e.g., oligosaccharides and dietary fibers) in raising colon Bifidobacterium population. In conclusion, our findings provide an insight into novel applications of an acid protease derived from Aspergillus as a functional food supplement for colon health.

Guangjian Jiang

Beijing University of Chinese Medicine, China

Title: Ginsenoside Rb1 promotes browning through regulation of PPARγ in 3T3-L1 adipocytes

Biography:

Guangjian Jiang has received her BS from North China Coal Medical University and PhD in Medical Science from Hebei Medical University. She has completed Post doctorate training in State Key Laboratory of Reproductive Biology, The Institute of Zoology of Chinese Academy of Sciences and worked as a Post Doctor at Harvard University Medical School Beth Israel Deaconess Medical Center in Boston. She has worked as a Dean of Department of Biochemistry and Molecular Biology in North China University of Science and Technology. Currently, she is a Professor in Diabetes Research Center, Beijing University of Chinese Medicine. Her research interests include natural medicine (traditional Chinese medicine) treatment of chronic disease and majored in molecular and cellular mechanisms of diabetes, cancer and age-associated degenerative diseases. She has focused on the Epigenetics, Traditional Chinese Medicine and Reproductive Biology areas.

Abstract:

Browning of white adipocyte tissue (WAT) has received considerable attention due to its potential implication in preventing obesity and related comorbidities. Ginsenoside Rb1 is reported to improve glycolipid metabolism and reduce body weight in obese animals. However whether the body reducing effect mediates by browning effect remains unclear. For this purpose, 3T3-L1 adipocytes were used to study the effect of ginsenoside Rb1 on browning adipocytes specific genes and oxygen consumptions. The results demonstrate that 10 mM of ginsenoside Rb1 increases basal glucose uptake and promoted browning evidenced by significant increases in mRNA expressions of UCP-1, PGC-1a and PRDM16 in 3T3-L1 mature adipocytes. Further, ginsenoside Rb1 also increases PPARγ activity. And the browning effect is abrogated by GW9692, a PPARγ antagonist. In addition, ginsenoside Rb1 increases basal respiration rate, ATP production and uncoupling capacity in 3T3-L1 adipocytes. Those effects are also blunted by GW9692. The results suggest that ginsenoside Rb1 promote browning of 3T3-L1 adipocytes through induction of PPARγ. Our finding offer a new source to discover browning agonists and also useful to understand and extend the applications of ginseng and its constituents.

Baodong Zheng

Fujian Agriculture and Forestry University, China

Title: Sensory and nutritional properties of chinese olive pumace based high fiber biscuit

Biography:

Baodong Zheng has completed his PhD from Fujian Agriculture and Forestry University. He is the Dean of College of Food Science, Fujian Agriculture and Forestry University. He has published more than 20 papers in reputed journals and has been serving as the Editorial Board Member of Journal of Toxicology.

Abstract:

Consumer interest in healthy eating is shifting towards the potential health benefits of specific foods and food ingredients. Unconventional ingredients combined with usual bakery flour yields better acceptable, healthy and nutritious bakery products. The present study aims to evaluate the sensory and nutritional properties of Chinese olive pumace based high fiber biscuit. The high fiber biscuit was formulated using Chinese olive pumace powder (15%), wheat (100 g), sugar powder (33%) and spices after repeated trials and the recipe was standardized. When subjected to sensory evaluation, the newly formulated high fiber biscuit derived a score of 4.5±0.51, out of a maximum score of 5, for its overall acceptability, indicating the biscuit was well accepted. The results of the nutritional quality and cost of the newly formulated high fiber biscuit showed that it contains the 10.20 g fiber, 1454 Kcal calories and 18.30 g fat in 100 g biscuit. Furthermore, the glycemic index (GI) results indicated that GI of olive high fiber biscuits was 63.68 which was significantly lower than the control one with the GI value of 76.66. Besides, the physical and chemical indicators as well as the microbiological indicators were also up to the Chinese national standards (GB/T20980-2007 and GB7100-2003). Hence, the study proved that the newly formulated olive pumace powder based biscuit is well acceptable based on its sensory properties and fiber rich nutritional quality.

Baodong Zheng

Fujian Agriculture and Forestry University, China

Title: Sensory and nutritional properties of chinese olive pumace based high fiber biscuit

Biography:

Baodong Zheng has completed his PhD from Fujian Agriculture and Forestry University. He is the Dean of College of Food Science, Fujian Agriculture and Forestry University. He has published more than 20 papers in reputed journals and has been serving as the Editorial Board Member of Journal of Toxicology.

Abstract:

Consumer interest in healthy eating is shifting towards the potential health benefits of specific foods and food ingredients. Unconventional ingredients combined with usual bakery flour yields better acceptable, healthy and nutritious bakery products. The present study aims to evaluate the sensory and nutritional properties of Chinese olive pumace based high fiber biscuit. The high fiber biscuit was formulated using Chinese olive pumace powder (15%), wheat (100 g), sugar powder (33%) and spices after repeated trials and the recipe was standardized. When subjected to sensory evaluation, the newly formulated high fiber biscuit derived a score of 4.5±0.51, out of a maximum score of 5, for its overall acceptability, indicating the biscuit was well accepted. The results of the nutritional quality and cost of the newly formulated high fiber biscuit showed that it contains the 10.20 g fiber, 1454 Kcal calories and 18.30 g fat in 100 g biscuit. Furthermore, the glycemic index (GI) results indicated that GI of olive high fiber biscuits was 63.68 which was significantly lower than the control one with the GI value of 76.66. Besides, the physical and chemical indicators as well as the microbiological indicators were also up to the Chinese national standards (GB/T20980-2007 and GB7100-2003). Hence, the study proved that the newly formulated olive pumace powder based biscuit is well acceptable based on its sensory properties and fiber rich nutritional quality.

Session Introduction

Muhammad Umair Arshad

University of Toronto, Canada

Title: Acute effects of different dietary polysaccharides added in milk on food intake, postprandial appetite and glycemic responses in healthy young females

Biography:

Muhammad Umair Arshad is from University of Toronto, Canada

Abstract:

Dietary polysaccharides may contribute to metabolic and physiological regulations, including satiety and glycemia, because of their properties of adding bulk and producing viscosity. In the present study we compared the postprandial glycemic and satiety responses of different dietary polysaccharides when added in milk (2% M.F.). The objective of this study was to evaluate the potential of different polysaccharides against postprandial glucose, appetite responses and food intake at subsequent meal. In a repeated measures crossover trial, 30 female participants (18-30 years) randomly consumed 250 ml milk 2% M.F. (control), or milk containing carrageenan (2.5 g), guar gum (2.5 g) and alginate (2.5 g). An ad libitum pizza meal was served to measure the food intake at 120 min following the treatments. Alginate and guar gum addition resulted in lower food intake as well as cumulative energy intake at subsequent pizza meal compared with control treatment. The post-treatment (0-120 min) as well as cumulative (0-170 min) postprandial glucose levels and average appetite scores were also significantly suppressed following alginate and guar gum compared with control (P<0.0001) with more pronounced effect of guar gum during post-treatment time (0-120 min). However, alginate resulted in significantly lower blood glucose mean values (P<0.0001) compared with control as well as carrageenan during post-treatment (0-120 min) and cumulative periods (0-170 min). In Conclusion, addition of polysaccharides in milk particularly, alginate and guar gum would be beneficial in the short term regulation of postprandial glycemia and satiety, respectively.

Kiarn Thakur

National Dairy Research Institute, India

Title: Riboflavin producing probiotic lactobacilli as a biotechnological strategy to obtain riboflavin enriched functional foods

Biography:

Kiarn Thakur is from National Dairy Research Institute, India

Abstract:

Riboflavin is an obligatory component of cellular metabolism, being as ultimate precursor of coenzyme FMN and FAD which are obtained by the phosphorylation of riboflavin in all living cells. It has been traditionally synthesized for food and feed fortification by chemicals means but past decade has witnessed a surge in information about commercial biotechnological processes. Hence this project was aimed at the isolation, identification and riboflavin operon characterization of lactobacilli from various niches. Among the 55 isolates bioprospected from dairy and non dairy sources, 16 isolates were found harboring complete Rib structural genes. The cloning and sequencing of rib genes from one isolates was done for BLAST analysis. The isolates harboring both complete as well as incomplete operon were compared phenotypically for riboflavin production by chemical, fluorescence and microbiological based assays and the microbiological assay method was found most sensitive among these three methods. Among the 30 isolates tested for riboflavin production ability, 10 were found to be riboflavin producers. Among them, isolates viz., KTLF1, KTLF9, KTLP1, KTLF11, and KTLF16 have shown 1.89 mg/L, 1.5 mg/L, 1.456 mg/L, 1.19 mg/L and 1.67 mg/L respectively. The isolate KTLF12, LP13 and KTLF2 have shown 0.95 mg/L, 0.83 mg/L and 0.46 mg/L of riboflavin production. (These isolates were able to survive in medium devoid of riboflavin as well as they have supported the growth of riboflavin auxotroph (L. casei MTCC1408). Among the screened isolates on agar diffusion assay, the maximum increase in growth of auxotroph was observed in the presence of KTLF1 and KTLF16 respectively. Expression pattern of rib genes was studied in selected isolates viz., LF1, LF2, LF3, LF4, LP1 and MTCC8711. RNA was isolated at different intervals of time in MRS and Riboflavin assay medium (RAM), milk and whey based medium. The range of relative fold in mRNA expression in Rib1 gene is 5 to 55 fold, 0.5 to 35 fold in Rib2 gene, 0.2 to 6.5 fold in Rib3 and 0.2 to 26 fold in Rib4 in MRS and RAM over control culture. On the basis of fold increase in relative mRNA expression of all the Rib genes, the isolate KTLF1 was selected for expression studies in milk and whey. The fold increase observed was 0 to 1.1 fold in Rib1, 0 to 2 fold in Rib2, 0 to 2 fold in Rib 3 and 0 to 0.9 fold increases in mRNA expression in milk and whey. The riboflavin producers were further screened for in vitro probiotic, safety aspects as well as technological properties. Three riboflavin producing isolates KTLF2, KTLF5 and KTMUC were able to show potential probiotic and safety attributes, while KTLF5 was showing appreciable adhesion on HT-29 cell lines as well as hold the promises to be used as novel starter cultures. The expression profile has given the clear picture of variation in expression profile of rib genes at different intervals of time. All of the four genes have displayed significant difference with respect to media and time intervals. The isolate KTLF1, KTLP1 from human feces, KTLF16 from fermented bamboo shoot has shown highest riboflavin production. The study has generated the data for further exploration of these isolates endowed with appreciable starter as well as functional activities for industrial use as novel and native starter cultures to produce an essential vitamin in situ which would contribute significantly to the functional value of certain fermented foods.

Natanamurugaraj Govindan

Universiti Malaysia Pahang, Malaysia

Title: The clinical trials of Au and Ag nanoparticles biosynthesized using Commelina nudiflora L. against the functional mechanism of colon cancer

Biography:

Natanamurugaraj Govindan has completed his PhD from Bharathidasan University, India and Postdoctoral studies from Massey University, School of Engineering and Advanced Technology, New Zealand. He has published more than 35 papers in reputed journals and has been serving as an Editorial Board Member of International Journal of Chemical and Life Sciences, International Journal of Medicine and Biosciences, Journal of Research and Application and International Journal of Diabetes & Clinical Research. The current research areas of Nutraceutical treatment for colon cancer and anticancer activity from microalgae.

Abstract:

Commelina nudiflora L. aqueous extract as a reducing and stabilizing agent for the synthesis of metallic gold and silver nanoparticles in green chemistry approaches. The biosynthesized gold and silver nanoparticles have studied their physicochemical and biological properties in nanoscale regime. The synthesized gold and silver nanoparticles physicochemical properties were characterized by various analytical techniques such as UV-VIS, FESEM, XRD and FT-IR. The outcome shows that the physicochemical characterizations of gold and silver nanoparticles were crystalline in nature with size range between 25-45 nm and 50-150 nm respectively. The EDX spectrum was shown strong signal energy peaks of both gold and silver atoms in 2-3 keV. The in vitro antioxidant ability of C. nudiflora extracts were studied using DPPH and ABTS radical scavenging assay. Moreover the C. nudiflora plant extract synthesized gold and silver metal nanoparticles have significantly control the proliferation of HCT-116 colon cancer cells in in vitro. The biosynthesized gold and silver nanoparticles were showed reduced cell viability and increased cytotoxicity on HCT-116 colon cancer cells with IC50 concentration of 200 and 100 µg/ml. Furthermore, the flow cytometry experiments showed that the IC50 concentrations of gold and silver nanoparticles treated cells are increased DNA fragmentation and significant changes were observed in sub G1, S and G2 cell cycle phases compared with positive control. Additionally, the mRNA gene expressions of HCT-116 cells were studied by RT-qPCR techniques. The pro-apoptotic genes are highly expressed in the gold nanoparticles treated HCT-116 colon cancer model. However, the C. nudiflora extract as a novel source for synthesis of metallic gold and silver nanoparticles with controlled size and shapes and also it could be potent anti-colon cancer drug in the near future.

Session Introduction

Muhammad Umair Arshad

University of Toronto, Canada

Title: Acute Effects Of Different Dietary Polysaccharides Added In Milk On Food Intake, Postprandial Appetite And Glycemic Responses In Healthy Young Females

Biography:

Muhammad Umair Arshad is from University of Toronto, Canada

Abstract:

Dietary polysaccharides may contribute to metabolic and physiological regulations, including satiety and glycemia, because of their properties of adding bulk and producing viscosity. In the present study we compared the postprandial glycemic and satiety responses of different dietary polysaccharides when added in milk (2% M.F.). The objective of this study was to evaluate the potential of different polysaccharides against postprandial glucose, appetite responses and food intake at subsequent meal. In a repeated measures crossover trial, 30 female participants (18-30 years) randomly consumed 250 ml milk 2% M.F. (control), or milk containing carrageenan (2.5 g), guar gum (2.5 g) and alginate (2.5 g). An ad libitum pizza meal was served to measure the food intake at 120 min following the treatments. Alginate and guar gum addition resulted in lower food intake as well as cumulative energy intake at subsequent pizza meal compared with control treatment. The post-treatment (0-120 min) as well as cumulative (0-170 min) postprandial glucose levels and average appetite scores were also significantly suppressed following alginate and guar gum compared with control (P<0.0001) with more pronounced effect of guar gum during post-treatment time (0-120 min). However, alginate resulted in significantly lower blood glucose mean values (P<0.0001) compared with control as well as carrageenan during post-treatment (0-120 min) and cumulative periods (0-170 min). In Conclusion, addition of polysaccharides in milk particularly, alginate and guar gum would be beneficial in the short term regulation of postprandial glycemia and satiety, respectively.

Session Introduction

Rakesh K Trivedi

professor & Director STEP-HBTI (Harcourt Butler Technological Institute), India

Title: Lipid based Nutraceuticals: availability and acceptance

Time : 11:00-11:25

Biography:

Prof (Dr) Rakesh K. Trivedi, M.Tech. & Ph.D., is currently Professor at HBTI,Kanpur & Director STEP-HBTI as well. He is a Teaching Faculty in the Department of Oil & Paint Technology at HBTI, Kanpur for 31 Years. He specializes in the area of lipids has published more than 65 research papers in national and international journals, supervised three doctoral theses and edited Five books. He has worked on Two visiting assignments at University of Guelph, Canada and Purdue University, USA, chaired several international seminars. He is the present Vice-President of Oil Technologist Association of India (HQ).He has worked as consultant to MI, WHO, CPCB, MARKFED, HAFED.

Abstract:

Nutraceutical based on lipids are derived mainly from oil seeds. Several fatty acids,which are derived from fatty oils are and part of food products , are dietary supplements. These lipid based fatty acids can promote our health or provide protection against some diseases. Based on several studies it has been found that long chain polyunsaturated fatty acids (LCPUFA) and some fatty acids with conjugated double bonds would fall in this category. Polyunsaturated fatty acids contain two or more double bonds and have long been recognized as essential nutrients for mammals. Docosahexaenoic acid (DHA), have been found in the cells of mammalian retina and brain tissues, are essential for their growth and normal function of human eye and brain. PUFA have been recognized as beneficial for patients who suffer from few diseases. Conjugated fatty acids are newly recognized nutraceutical compounds, within which, conjugated linoleic acid (CLA) is presently the most popular. Dietary supplements of this fatty acid was shown to inhibit chemical induced skin and stomach cancers, reduce development to atherosclerosis and enhance immune function in mammals. There are hundreds of different lipid based compounds identified in nature, of which many have potentials for nutraceutical and pharmaceutical uses. The details presentation will be given along with availability of these componds.

Thanutchaporn Kumrungsee

Hiroshima University, Japan

Title: A novel bioactive compound derived from yeast extract as a potent nutraceutical for preventing vascular-related diseases

Biography:

Thanutchaporn Kumrungsee has completed her BS in Food Science and Technology from Kasetsart University, Thailand, MS and PhD in Food Analysis and Postdoctoral studies from Kyushu University Japan. Currently, she is an Assistant Professor in Laboratory of Molecular Nutrition at Graduate School of Bioshere Science, Hiroshima University, Japan. Her research interests are in elucidation of food factors with potent preventive effects on lifestyle-related diseases and their mechanisms by application of metabolomics analyses. Her inspirational research quote is “Let food be your medicine”.

Abstract:

Yeast extract has been used commercially in a food industry as a food additive. In spite of rich in nutrition, an application as nutraceuticals and functional foods along with its physiological effects is not wildly studied.　Since it was reported that compounds containing a purine moiety e.g., adenine and adenosine and an imidazole moiety e.g., bioactive Trp-His peptide, exerted vascular protective effects such as vasorelaxant, anti-hypertensive and atherosclerotic effects. Thus, it was speculated that yeast extract rich in nucleotides might mediate vasoprotective effects and contain the potent candidates’ compounds. The objectives of this study were to examine the vasorelaxant effect of yeast extract (Candida utilis); identify candidates responsible for the effect by using chromatographic and mass spectrometric analyses; and elucidate the underlying mechanisms. This study provided the first evidence that C. utilis yeast extract exerted vasorelaxation in 1 μM phenylephrine (PE)-contracted Sprague-Dawley rat aortic rings. 5'-Methylthioadenosine (MTA) was identified as a new naturally-occurring vasodilator, exerting comparable power with a well-known vasodilator adenosine. MTA exerted vasorelaxation independent of endothelial layer and adenosine receptors. MTA reduced a CaCl2-induced vasocontraction stimulated by 1 μM PE, whereas the effect was abolished in a 60 mM KCl-induced vasocontraction. MTA significantly (P<0.01) attenuated the PE-stimulated calmodulin-dependent kinase II (CaMK II) in the aortic rings and inhibited the phosphorylation of L-type Ca2+ channel (VDCC). In conclusion, the underlying mechanisms of MTA-induced vasorelaxation involve the suppression of extracellular Ca2+ influx partly through retardation of the CaMK II-VDCC phosphorylation pathway.

Jatin R Joshi

Founder & President Instavit Ltd, London, UK

Title: It’s not the innovation, it’s the application: How vitamins saved my life and career

Biography:

Jatin R Joshi has completed his Medical training from University College London, graduating with honors. He did his Surgical Residency in London with specialization in Plastic and Reconstructive surgery gaining Membership of the Royal College of Surgeons. He is the Founder and President of Instavit Ltd, a nutraceutical company that utilizes oral spray technology as the delivery vehicle for nutritional supplementation. He has published several papers, authored surgical textbooks and presented internationally.

Abstract:

The need for nutritional supplementation is a contentious issue. It is one that has divided both the scientific community and public opinion. With my background as a surgeon I started as a cynic, I would see undigested tablets in the colons of patients and ingredient lists full of indigestible fillers. My opinion was that in a society where a healthy and well-balanced diet was accessible to most, there was no need to consider vitamin supplementation. All this changed after I was diagnosed with Crohn’s Disease in my early twenties. I was a trainee surgeon working over 110 hours a week whilst my condition progressively deteriorated. Following major emergency abdominal surgery, I was left with severe nutritional deficiencies; the need to take vitamin supplements became essential to my well being, but as with most individuals, my compliance rates were appalling. I had to find another way. The journey for me from a surgeon to an innovator was driven out of a genuine necessity, not only to find a way to increase my compliance but also to help others struggling to achieve a balanced diet or those having difficulty with digestive absorption issues. Approaching the problems from a functional and delivery standpoint, I was able to develop a line of supplements that use oral spray technology to improve compliance and dosage control by way of its delivery system, accessibility and ease of use. It was the answer for me but as I discovered it became the answer for thousands of others.

Seung-Kwon Myung

National Cancer Center, South Korea

Title: Efficacy of vitamin and antioxidant supplements in prevention of bladder cancer: Meta analysis of randomized controlled trials

Biography:

Seung-Kwon Myung has received his MD from Seoul National University College of Medicine and has completed his PhD in Family Medicine from the same university. He is an Associate Professor of Graduate School of Cancer Science and Policy, National Cancer Center, Korea. He has published 63 papers in reputed medical journals and served as an Editor-In-Chief of the Korean Journal of Family Medicine. His research specialty is meta-analysis and his research areas are cancer prevention and food, specifically the efficacy of vitamin supplements and functional foods on health.

Abstract:

This meta-analysis aimed to investigate the efficacy of vitamin and antioxidant supplements in the prevention of bladder cancer as reported by randomized controlled trials (RCTs). We searched PubMed, EMBASE and the Cochrane Library in April 2015. Two of the authors independently reviewed and selected eligible RCTs, based on pre-determined selection criteria. Out of 180 articles searched from three databases and relevant bibliographies, 14 RCTs were included in the final analysis. In a fixed effect meta-analysis, there was no efficacy of vitamin and antioxidant supplements in the prevention of bladder cancer (relative risk (RR), 1.03; 95% confidence interval (CI), 0.91-1.16; I2=43.4%). Overall, there was no preventive effect of these supplements in the subgroup meta-analyses by various factors. Among the subgroup analyses by type of supplements, beta-carotene supplementation alone was marginally increased the risk of bladder cancer (RR, 1.44; 95% CI, 1.00-2.09; I2=0.0%; n=3). The current meta-analysis of RCTs found that there was no clinical evidence to support the efficacy of vitamin and antioxidant supplements in the prevention of bladder cancer.

Kiran Thakur

National Dairy Research Institute, India

Title: Riboflavin producing probiotic lactobacilli as a biotechnological strategy to obtain riboflavin enriched functional foods

Biography:

Dr Kiran Thakur has completed Ph.D in Dairy Microbiology at National Dairy Research Institute (NDRI), Karnal, and Haryana, India. She is the member of Singapore Society for Microbiology and Biotechnology (SSMB) and International Scientific Association for Probiotics and Prebiotics (ISAPP). She has been conferred BEST THESIS AWARD 2013-2014 for outstanding Doctoral Research work and Director’s Gold Medal for the same. Her PhD work has been recognized at various national and international platforms. She has attended Annual meeting organized by (ISAPP) at Georgetown University, Washington DC, USA (May 18- 21, 2015) and International Seminar on Pharmaceutical Science and Technology at Padjadjaran University at Indonesia. She has been granted “Outstanding Oral Presenter” at 7th Asian Conference at Lactic Acid Bacteria held on 6- 8 September, 2013 at Indian habitat centre, New Delhi and also bagged Best Poster Presentation Award at National level. She has Presented Poste for Rowett-INRA 2014 Conference (co-hosted by ISAPP) “Gut Microbiology: from sequence to function”, at Aberdeen, Scotland, UK on 16-19 June, 2014. She has attended workshop at National University of Singapore Sep, 2013. She has published 9 Research and Review articles with high impact international and national journals. She has published 12 popular articles. She has submitted 15 partial 16s DNA sequences in NCBI. She is the authors of many articles in newspapers and magazines. She has also contributed for technical manuals and compendium at national levels. She has authored two books for Lambert Academic Publishing, Germany. She is continuously seeking a dynamic & challenging career in Academics as well as Research and development in Food science and Technology and to promote the new research scenario as an ideal human resource.

Abstract:

Riboflavin is an obligatory component of cellular metabolism, being as ultimate precursor of coenzyme FMN and FAD which are obtained by the phosphorylation of riboflavin in all living cells. It has been traditionally synthesized for food and feed fortification by chemicals means but past decade has witnessed a surge in information about commercial biotechnological processes. Hence this project was aimed at the isolation, identification and riboflavin operon characterization of lactobacilli from various niches. Among the 55 isolates bioprospected from dairy and non dairy sources, 16 isolates were found harboring complete Rib structural genes. The cloning and sequencing of rib genes from one isolates was done for BLAST analysis. The isolates harboring both complete as well as incomplete operon were compared phenotypically for riboflavin production by chemical, fluorescence and microbiological based assays and the microbiological assay method was found most sensitive among these three methods. Among the 30 isolates tested for riboflavin production ability, 10 were found to be riboflavin producers. Among them, isolates viz., KTLF1, KTLF9, KTLP1, KTLF11, and KTLF16 have shown 1.89 mg/L, 1.5 mg/L, 1.456 mg/L, 1.19 mg/L and 1.67 mg/L respectively. The isolate KTLF12, LP13 and KTLF2 have shown 0.95 mg/L, 0.83 mg/L and 0.46 mg/L of riboflavin production. (These isolates were able to survive in medium devoid of riboflavin as well as they have supported the growth of riboflavin auxotroph (L. casei MTCC1408). Among the screened isolates on agar diffusion assay, the maximum increase in growth of auxotroph was observed in the presence of KTLF1 and KTLF16 respectively. Expression pattern of rib genes was studied in selected isolates viz., LF1, LF2, LF3, LF4, LP1 and MTCC8711. RNA was isolated at different intervals of time in MRS and Riboflavin assay medium (RAM), milk and whey based medium. The range of relative fold in mRNA expression in Rib1 gene is 5 to 55 fold, 0.5 to 35 fold in Rib2 gene, 0.2 to 6.5 fold in Rib3 and 0.2 to 26 fold in Rib4 in MRS and RAM over control culture. On the basis of fold increase in relative mRNA expression of all the Rib genes, the isolate KTLF1 was selected for expression studies in milk and whey. The fold increase observed was 0 to 1.1 fold in Rib1, 0 to 2 fold in Rib2, 0 to 2 fold in Rib 3 and 0 to 0.9 fold increases in mRNA expression in milk and whey. The riboflavin producers were further screened for in vitro probiotic, safety aspects as well as technological properties. Three riboflavin producing isolates KTLF2, KTLF5 and KTMUC were able to show potential probiotic and safety attributes, while KTLF5 was showing appreciable adhesion on HT-29 cell lines as well as hold the promises to be used as novel starter cultures. The expression profile has given the clear picture of variation in expression profile of rib genes at different intervals of time. All of the four genes have displayed significant difference with respect to media and time intervals. The isolate KTLF1, KTLP1 from human feces, KTLF16 from fermented bamboo shoot has shown highest riboflavin production. The study has generated the data for further exploration of these isolates endowed with appreciable starter as well as functional activities for industrial use as novel and native starter cultures to produce an essential vitamin in situ which would contribute significantly to the functional value of certain fermented foods.

Prachya Kongtawelert

Chiang Mai University, Thailand

Title: Sesamin: From sesame to the functional foods

Biography:

Prachya Kongtawelert has completed his PhD from Sydney University since 1992. He is currently working as Associate Professor in Department of Biochemistry. He is interested in researches concerning about pathogenesis of osteoarthritis, rheumatoid arthritis, including diagnosis and treatment. Further, he has developed the formulation for dietary supplement products based on the active ingredient of sesamin, which is a natural compound from sesame.

Abstract:

Sesame has been used as food over 4,000 years. It contains very high value of nutritional compounds. One of them is sesamin, which is the most containing of lignan in sesame. There are many research papers concerning about its pharmacological activities. This leading to the research, and development, and apply this natural compound, sesamin, for widely uses as functional foods and cosmetics. It has been studied in our laboratorie as anti-cartilage degradation (as anti-osteoarthritis and anti-rheumatoid arthrisits) by inhibition of the Inteleukin-1 Beta, which is the most potent pro-inflammatoray cytokine, throgh NFkB and NAPK sinalling pathway in human chondrocyte culture. Sesamin shows the chodroprotective effects in cartilage explant culture and papain induced cartilage degradation in rat model. We are also studied anti-osteoporosis as enhancing the mineralisation, and stimulating osteoblast functions by up-regulating Type-II Collagen, alkaline phosphatase, and bone morphogenetic protein-2 (BMP-2). The osteoclastogenesis was inhibited by sesamin. Furthermore, the neuro-protective effect on the LPS-induced neuronal cell (PC12) development has been studied. Finally, the pathway of commercialization for development of simple and innovative dietary supplement of healthy products from sesamin with other natural resources, such as black rice bran and pre-cooked rice have been succesfully formulated and sold in several countries, especially in Southeast Asian.

Pritee Paliwal

Synthite Industries Ltd, India

Title: Technology Next-Generation Nutraceutical world

Time : 13:55-14:20

Biography:

Dr Pritee Paliwal has as over 15 years experience in Neutraceutical and Personal care Industry. She acquired a US Patent (US 2011/0033565) of Cosmo-Technology. She is also the Co-Author for LLC Nutriscience Publication, Tetrahydrocurcuminoids (C3 Reduct –Cascade Transformation. Globally she presented technical seminar and launched products- UK, Germany, Switzerland, Brazil, Barcelona, Bangkok, Taiwan, South Korea, China, India. She is the Heading Nutraceutical team of Synthite® Industries Ltd., is the world’s largest manufacturer and exporter of Spice Oleoresin and essential oils..She also won young scientist award and has authored 15 international publications and 12 articles in magzines world wide.

Abstract:

The current study aims to identify safe technology for nutraceutical ingredients. Supercritical fluids are highly compressed gases, which have combined properties of gases and liquids in an intriguing manner. Supercritical fluids can lead to reactions, which are difficult or even impossible to achieve in conventional solvents. It is a fast process completed in 10 to 60 minutes. A supercritical fluid can be separated from analyte by simply releasing pressure, leaving almost no trace and yields a pure residue. Straights place the goodness of minimally processed artisan ingredients in the hands of the Formulator and Food Manufacturer. When you create your next line of seasonings, soups, chorizos, pepperonis, sauces salads, bakes, goulashes, curries, salsa’s and stews, our straight extracts will add that perfect natural flavor and color making your products the choice of the consumer. Nutraceuticals are products that provide health and medicinal benefits, including the prevention and treatment of disease in addition to the basic nutritional value found in foodstuff. Nutraceuticals are particularly of interest to the present generation because they have the potential to substantially reduce the expensive, high tech, disease treatment approaches presently being employed in western healthcare. Primarily used in functional foods and dietary supplements, nutraceuticals ingredients are natural bioactive, chemical compounds that have health promoting disease preventive or medical properties. Since the early 1990s, there has been a considerable shift in consumers (Especially consumers from developed countries) perspective towards nutraceuticals and functional foods. Currently, consumers are much more conscious and aware about health and many share the perception that the onset of many chronic diseases can be prevented with the proper intake of nutritious diet. Food supplements are not only being consumed for just meeting the recommended dietary allowances but also as a mechanism for performance enhancement and disease prevention. An accelerated world demands an accelerated lifestyle. This in turn has placed huge demands on our health, which is reflected in our food habits. While we may have no control over our lives, we can control our dietary behavior. This is where science and technology meets food and nutrition. The increased public awareness of the health, environment and safety concerns associated with the use of some organic solvents in food processing and the possible solvent contamination of the final products. The high cost of organic solvents and the increasingly stringent environmental regulations together with the new requirements of the medical and food industries for ultra-pure and high added value products have pointed out the need for the development of new and clean technologies for the processing of food ingredients. Supercritical fluid extraction (SCFE) process has emerged as the alternative to the traditional solvent extraction process. It is a separation technique, which is developed by use of the fluid that has the ability of dissolution at supercritical pressure and supercritical temperature. Synthite® has a state-of-the-art manufacturing plant for new technology (Super Critical Fluid Extraction (CO2) and Straight (Straights are a class of 100% natural non-selective, mechanically processed ingredients), which ensures green, environment-friendly isolates of the botanical, by eliminating solvent residue and high temperature processing. Green technology is the answer to the growing demand for pure and natural substances in the industry and the Straights are our answer to this multidimensional challenge that formulators and manufacturers face

Siew Young Quek

The University of Auckland, New Zealand

Title: Microencapsulation of bioactives for functional food development

Biography:

Siew Young Quek was graduated with a PhD degree from School of Chemical Engineering, University of Birmingham (UK) in 1999. She is the Director of Food Science Program at University of Auckland. Her current research interests include microencapsulation of bioactives for delivery into functional foods, extraction & characterization of bioactives/functional ingredients from food and waste byproducts, processing & food quality and food product development. She has published more than 200 journals articles and conference proceedings, 4 book chapters and delivered 21 reports for industries. She is a Fellow of the New Zealand Institute of Food Science and Technology.

Abstract:

The increasing pressure on the food industry to produce healthy food products has, as a result, unveiled the challenge of processing functional ingredients in a way that meets the requirements of the modern day consumer. The major issues limiting the use of functional ingredients without prior processing include vulnerability to oxidative breakdown, limited water or lipid solubility, poor thermal stability and poor taste. Unprocessed, these ingredients such as bioactive compounds are essentially unsuitable for use as food ingredients due to any of the aforementioned reasons. This leads to the application of microencapsulation technology as one of the methods in production of food ingredients for functional food application. By utilizing microencapsulation technology, it is possible to produce functional ingredients with desirable chemical and physical properties. This paper aims to provide an understanding on the application of microencapsulation processes to produce functional ingredients for foods. Microencapsulation of lipophilic bioactive components (LBCs) and the development in the area will be discussed.

Toshikazu Suzuki

Wayo Women’s University, Japan

Title: Changes in blood coenzyme Q10 levels by food intervention and intake of CoQ10-fortified rice

Biography:

Toshikazu Suzuki has completed his PhD at the age of 27 years from Graduate School of Pharmaceutical Sciences, Chiba University and postdoctoral studies from National Institute of Infectious Disease, Japan. He is the Associate Professor of Department of Health and Nutrition, Wayo Women’s University. He has published more than 60 papers in Journals and has been serving as a councilor of The Japanese Clinical Nutrition Association.

Abstract:

A non-essential nutrient, coenzyme Q10 (CoQ10) is a key element in mitochondrial energy production and antioxidant protection. Daily intake of CoQ10 is not considered in nutritional guidance or menu planning. Hospitalized older people have lower blood levels of CoQ10 with possible decreased intake of CoQ10 compared with healthy older people, suggesting that adequate intake of CoQ10 maintains wellness in older people. First we estimated daily intake of CoQ10 from food, designed a food intake guide for ingestion of increased amounts of CoQ10 with balanced food choice and evaluated the usability in a diet intervention trial. Average daily intake of CoQ10 from food was 1.9 mg/1000 kcal/day in both men and women. Ratio of dietary animal to vegetable protein was involved in the amount of CoQ10 intake. Our food intervention was effective in increasing CoQ10 intake at up to 1mg/day while maintaining PFC balance. However, choice of food items was sometimes a burden to the participants. Next, we investigated the effect of food choice and efficacy of CoQ10-fortified food on blood CoQ10 levels. Two weeks prohibition of meat/poultry consumption decreased blood CoQ10 levels by ~0.1µg/mL. Eating 300 g/day of CoQ10-fortified boiled rice (13 mg CoQ10/100g rice) could increase both intake and blood levels of CoQ10. Our results indicate that the choice of a CoQ10-fortified food may be more applicative for keeping/raising blood CoQ10 levels than food intervention. Further study of the effect of CoQ10-fortified food intake on maintaining/improving the quality of life of the older people should be pursued

Muhammad Umair Arshad

University of Toronto, Canada

Title: Acute effects of different dietary polysaccharides added in milk on food intake, postprandial appetite and glycemic responses in healthy young females

Biography:

Muhammad Umair Arshad is from University of Toronto, Canada

Abstract:

Dietary polysaccharides may contribute to metabolic and physiological regulations, including satiety and glycemia, because of their properties of adding bulk and producing viscosity. In the present study we compared the postprandial glycemic and satiety responses of different dietary polysaccharides when added in milk (2% M.F.). The objective of this study was to evaluate the potential of different polysaccharides against postprandial glucose, appetite responses and food intake at subsequent meal. In a repeated measures crossover trial, 30 female participants (18-30 years) randomly consumed 250 ml milk 2% M.F. (control), or milk containing carrageenan (2.5 g), guar gum (2.5 g) and alginate (2.5 g). An ad libitum pizza meal was served to measure the food intake at 120 min following the treatments. Alginate and guar gum addition resulted in lower food intake as well as cumulative energy intake at subsequent pizza meal compared with control treatment. The post-treatment (0-120 min) as well as cumulative (0-170 min) postprandial glucose levels and average appetite scores were also significantly suppressed following alginate and guar gum compared with control (P<0.0001) with more pronounced effect of guar gum during post-treatment time (0-120 min). However, alginate resulted in significantly lower blood glucose mean values (P<0.0001) compared with control as well as carrageenan during post-treatment (0-120 min) and cumulative periods (0-170 min). In Conclusion, addition of polysaccharides in milk particularly, alginate and guar gum would be beneficial in the short term regulation of postprandial glycemia and satiety, respectively.

Natanamurugaraj Govindan

Universiti Malaysia Pahang, Malaysia

Title: The clinical trials of Au and Ag nanoparticles biosynthesized using Commelina nudiflora L. against the functional mechanism of colon cancer

Biography:

Natanamurugaraj Govindan has completed his PhD from Bharathidasan University, India and Postdoctoral studies from Massey University, School of Engineering and Advanced Technology, New Zealand. He has published more than 35 papers in reputed journals and has been serving as an Editorial Board Member of International Journal of Chemical and Life Sciences, International Journal of Medicine and Biosciences, Journal of Research and Application and International Journal of Diabetes & Clinical Research. The current research areas of Nutraceutical treatment for colon cancer and anticancer activity from microalgae.

Abstract:

Commelina nudiflora L. aqueous extract as a reducing and stabilizing agent for the synthesis of metallic gold and silver nanoparticles in green chemistry approaches. The biosynthesized gold and silver nanoparticles have studied their physicochemical and biological properties in nanoscale regime. The synthesized gold and silver nanoparticles physicochemical properties were characterized by various analytical techniques such as UV-VIS, FESEM, XRD and FT-IR. The outcome shows that the physicochemical characterizations of gold and silver nanoparticles were crystalline in nature with size range between 25-45 nm and 50-150 nm respectively. The EDX spectrum was shown strong signal energy peaks of both gold and silver atoms in 2-3 keV. The in vitro antioxidant ability of C. nudiflora extracts were studied using DPPH and ABTS radical scavenging assay. Moreover the C. nudiflora plant extract synthesized gold and silver metal nanoparticles have significantly control the proliferation of HCT-116 colon cancer cells in in vitro. The biosynthesized gold and silver nanoparticles were showed reduced cell viability and increased cytotoxicity on HCT-116 colon cancer cells with IC50 concentration of 200 and 100 µg/ml. Furthermore, the flow cytometry experiments showed that the IC50 concentrations of gold and silver nanoparticles treated cells are increased DNA fragmentation and significant changes were observed in sub G1, S and G2 cell cycle phases compared with positive control. Additionally, the mRNA gene expressions of HCT-116 cells were studied by RT-qPCR techniques. The pro-apoptotic genes are highly expressed in the gold nanoparticles treated HCT-116 colon cancer model. However, the C. nudiflora extract as a novel source for synthesis of metallic gold and silver nanoparticles with controlled size and shapes and also it could be potent anti-colon cancer drug in the near future.

Session Introduction

Kiran Thakur

National Dairy Research Institute, India

Title: Riboflavin Producing Probiotic Lactobacilli As A Biotechnological Strategy To Obtain Riboflavin Enriched Functional Foods

Biography:

Kiran Thakur is from National Dairy Research Institute, India

Abstract:

Riboflavin is an obligatory component of cellular metabolism, being as ultimate precursor of coenzyme FMN and FAD which are obtained by the phosphorylation of riboflavin in all living cells. It has been traditionally synthesized for food and feed fortification by chemicals means but past decade has witnessed a surge in information about commercial biotechnological processes. Hence this project was aimed at the isolation, identification and riboflavin operon characterization of lactobacilli from various niches. Among the 55 isolates bioprospected from dairy and non dairy sources, 16 isolates were found harboring complete Rib structural genes. The cloning and sequencing of rib genes from one isolates was done for BLAST analysis. The isolates harboring both complete as well as incomplete operon were compared phenotypically for riboflavin production by chemical, fluorescence and microbiological based assays and the microbiological assay method was found most sensitive among these three methods. Among the 30 isolates tested for riboflavin production ability, 10 were found to be riboflavin producers. Among them, isolates viz., KTLF1, KTLF9, KTLP1, KTLF11, and KTLF16 have shown 1.89 mg/L, 1.5 mg/L, 1.456 mg/L, 1.19 mg/L and 1.67 mg/L respectively. The isolate KTLF12, LP13 and KTLF2 have shown 0.95 mg/L, 0.83 mg/L and 0.46 mg/L of riboflavin production. (These isolates were able to survive in medium devoid of riboflavin as well as they have supported the growth of riboflavin auxotroph (L. casei MTCC1408). Among the screened isolates on agar diffusion assay, the maximum increase in growth of auxotroph was observed in the presence of KTLF1 and KTLF16 respectively. Expression pattern of rib genes was studied in selected isolates viz., LF1, LF2, LF3, LF4, LP1 and MTCC8711. RNA was isolated at different intervals of time in MRS and Riboflavin assay medium (RAM), milk and whey based medium. The range of relative fold in mRNA expression in Rib1 gene is 5 to 55 fold, 0.5 to 35 fold in Rib2 gene, 0.2 to 6.5 fold in Rib3 and 0.2 to 26 fold in Rib4 in MRS and RAM over control culture. On the basis of fold increase in relative mRNA expression of all the Rib genes, the isolate KTLF1 was selected for expression studies in milk and whey. The fold increase observed was 0 to 1.1 fold in Rib1, 0 to 2 fold in Rib2, 0 to 2 fold in Rib 3 and 0 to 0.9 fold increases in mRNA expression in milk and whey. The riboflavin producers were further screened for in vitro probiotic, safety aspects as well as technological properties. Three riboflavin producing isolates KTLF2, KTLF5 and KTMUC were able to show potential probiotic and safety attributes, while KTLF5 was showing appreciable adhesion on HT-29 cell lines as well as hold the promises to be used as novel starter cultures. The expression profile has given the clear picture of variation in expression profile of rib genes at different intervals of time. All of the four genes have displayed significant difference with respect to media and time intervals. The isolate KTLF1, KTLP1 from human feces, KTLF16 from fermented bamboo shoot has shown highest riboflavin production. The study has generated the data for further exploration of these isolates endowed with appreciable starter as well as functional activities for industrial use as novel and native starter cultures to produce an essential vitamin in situ which would contribute significantly to the functional value of certain fermented foods.

Session Introduction

Natanamurugaraj Govindan

Universiti Malaysia Pahang, Malaysia

Title: The Clinical Trials Of Au And Ag Nanoparticles Biosynthesized Using Commelina Nudiflora L. Against The Functional Mechanism Of Colon Cancer

Biography:

Natanamurugaraj Govindan has completed his PhD from Bharathidasan University, India and Postdoctoral studies from Massey University, School of Engineering and Advanced Technology, New Zealand. He has published more than 35 papers in reputed journals and has been serving as an Editorial Board Member of International Journal of Chemical and Life Sciences, International Journal of Medicine and Biosciences, Journal of Research and Application and International Journal of Diabetes & Clinical Research. The current research areas of Nutraceutical treatment for colon cancer and anticancer activity from microalgae

Abstract:

Commelina nudiflora L. aqueous extract as a reducing and stabilizing agent for the synthesis of metallic gold and silver nanoparticles in green chemistry approaches. The biosynthesized gold and silver nanoparticles have studied their physicochemical and biological properties in nanoscale regime. The synthesized gold and silver nanoparticles physicochemical properties were characterized by various analytical techniques such as UV-VIS, FESEM, XRD and FT-IR. The outcome shows that the physicochemical characterizations of gold and silver nanoparticles were crystalline in nature with size range between 25-45 nm and 50-150 nm respectively. The EDX spectrum was shown strong signal energy peaks of both gold and silver atoms in 2-3 keV. The in vitro antioxidant ability of C. nudiflora extracts were studied using DPPH and ABTS radical scavenging assay. Moreover the C. nudiflora plant extract synthesized gold and silver metal nanoparticles have significantly control the proliferation of HCT-116 colon cancer cells in in vitro. The biosynthesized gold and silver nanoparticles were showed reduced cell viability and increased cytotoxicity on HCT-116 colon cancer cells with IC50 concentration of 200 and 100 µg/ml. Furthermore, the flow cytometry experiments showed that the IC50 concentrations of gold and silver nanoparticles treated cells are increased DNA fragmentation and significant changes were observed in sub G1, S and G2 cell cycle phases compared with positive control. Additionally, the mRNA gene expressions of HCT-116 cells were studied by RT-qPCR techniques. The pro-apoptotic genes are highly expressed in the gold nanoparticles treated HCT-116 colon cancer model. However, the C. nudiflora extract as a novel source for synthesis of metallic gold and silver nanoparticles with controlled size and shapes and also it could be potent anti-colon cancer drug in the near future.

Session Introduction

Ying Ma

Harbin Institute of Technology, China

Title: Binding Characteristics Of Curcumin To β-Lactoglobulin

Biography:

Professor Ying Ma has completed her PhD from Northeast Agricultural University and postdoctoral studies from Chinese Agricultural University. Now Dr. Ma, as a professor and doctor student’s supervisor, works in institute of food science and engineering of Harbin Institute Technology. She also is associate editor of an international journal《Dairy Science and Technology》(formerly le lait). Her research area mainly focuses on the food chemistry, food safety，the modifications of starch and plant protein, and bioprocess of agricultural food. As a coordinator, she jointed several national and local research projects. More than 248 peer- reviewed research papers were published in primary journals. She owned 21 authorized patents and 22 edited Chinese books and 5 peer-reviewed English books.chapters.

Abstract:

Curcumin (CCM) is a phenolic compound and has been shown to exhibit many bioactivities, but it is extremely limited in its application due to its insolubility in water. β-Lactoglobulin (β-Lg) is a major whey protein and has an ability to bind hydrophobic molecules because of its hydrophobic core. In this experiments, the binding of CCM to β-Lg was investigated. The results showed that binding CCM to β-Lg leads to a partial change in protein structure. CCM was bound respectively to two different sites of protein at pH 6.0 and pH 7.0 via hydrophobic interaction. CCM-β-Lg complex was formed by one molecule of protein combining with one molecule of CCM. The solubility of CCM was increased by 15954-fold through the complex. The β-Lg -CCM complex and CCM in complex had a good stability in the pH range of 2 to 8. The results of antioxidant experiments showed that the ABTS and hydroxyl radical scavenging capacity of CCM was reduced by binding with β- Lg, but the total reducing ability of CCM was improved.

Session Introduction

Prachya Kongtawelert

Chiang Mai University, Thailand

Title: Sesamin: From sesame to the functional foods

Biography:

Prachya Kongtawelert has completed his PhD from Sydney University since 1992. He is currently working as Associate Professor in Department of Biochemistry. He is interested in researches concerning about pathogenesis of osteoarthritis, rheumatoid arthritis, including diagnosis and treatment. Further, he has developed the formulation for dietary supplement products based on the active ingredient of sesamin, which is a natural compound from sesame

Abstract:

Sesame has been used as food over 4,000 years. It contains very high value of nutritional compounds. One of them is sesamin, which is the most containing of lignan in sesame. There are many research papers concerning about its pharmacological activities. This leading to the research, and development, and apply this natural compound, sesamin, for widely uses as functional foods and cosmetics. It has been studied in our laboratorie as anti-cartilage degradation (as anti-osteoarthritis and anti-rheumatoid arthrisits) by inhibition of the Inteleukin-1 Beta, which is the most potent pro-inflammatoray cytokine, throgh NFkB and NAPK sinalling pathway in human chondrocyte culture. Sesamin shows the chodroprotective effects in cartilage explant culture and papain induced cartilage degradation in rat model. We are also studied anti-osteoporosis as enhancing the mineralisation, and stimulating osteoblast functions by up-regulating Type-II Collagen, alkaline phosphatase, and bone morphogenetic protein-2 (BMP-2). The osteoclastogenesis was inhibited by sesamin. Furthermore, the neuro-protective effect on the LPS-induced neuronal cell (PC12) development has been studied. Finally, the pathway of commercialization for development of simple and innovative dietary supplement of healthy products from sesamin with other natural resources, such as black rice bran and pre-cooked rice have been succesfully formulated and sold in several countries, especially in Southeast Asian.

Toshikazu Suzuki

Wayo Women’s University, Japan

Title: Changes in blood coenzyme Q10 levels by food intervention and intake of CoQ10-fortified rice

Biography:

Toshikazu Suzuki has completed his PhD at the age of 27 years from Graduate School of Pharmaceutical Sciences, Chiba University and postdoctoral studies from National Institute of Infectious Disease, Japan. He is the Associate Professor of Department of Health and Nutrition, Wayo Women’s University. He has published more than 60 papers in Journals and has been serving as a councilor of The Japanese Clinical Nutrition Association.

Abstract:

A non-essential nutrient, coenzyme Q10 (CoQ10) is a key element in mitochondrial energy production and antioxidant protection. Daily intake of CoQ10 is not considered in nutritional guidance or menu planning. Hospitalized older people have lower blood levels of CoQ10 with possible decreased intake of CoQ10 compared with healthy older people, suggesting that adequate intake of CoQ10 maintains wellness in older people. First we estimated daily intake of CoQ10 from food, designed a food intake guide for ingestion of increased amounts of CoQ10 with balanced food choice and evaluated the usability in a diet intervention trial. Average daily intake of CoQ10 from food was 1.9 mg/1000 kcal/day in both men and women. Ratio of dietary animal to vegetable protein was involved in the amount of CoQ10 intake. Our food intervention was effective in increasing CoQ10 intake at up to 1mg/day while maintaining PFC balance. However, choice of food items was sometimes a burden to the participants. Next, we investigated the effect of food choice and efficacy of CoQ10-fortified food on blood CoQ10 levels. Two weeks prohibition of meat/poultry consumption decreased blood CoQ10 levels by ~0.1µg/mL. Eating 300 g/day of CoQ10-fortified boiled rice (13 mg CoQ10/100g rice) could increase both intake and blood levels of CoQ10. Our results indicate that the choice of a CoQ10-fortified food may be more applicative for keeping/raising blood CoQ10 levels than food intervention. Further study of the effect of CoQ10-fortified food intake on maintaining/improving the quality of life of the older people should be pursued.

Session Introduction

Zhao-Jun Wei

Hefei University of Technology, China

Title: The nutraceutical properties of polysaccharides sequentially extracted from peony seed dreg

Biography:

Zhaojun Wei has received his BS from Anhui Agricultural University (1993), MS from Chinese Academy of Agricultural Sciences (2001) and PhD from University of Science and Technology of China (2005). Currently, he is the distinguished Professor and Deputy Dean of School of Biotechnology and Food Engineering at Hefei University of Technology (HFUT). He also serves as the Chairman of the Agricultural and forestry specialty food processing industry technological innovation strategic alliance of Anhui province, Chairman of the Hefei Society for Nutrition, and a Member of the Chinese Society of Biochemistry and Molecular of Agriculture. His research focuses on the processing and function of nutraceuticals from the byproducts of Agriculture and forestry, e.g., DNJ from mulberry, Pectin from okra, polysaccharides from peony seed dreg and others.

Abstract:

Peony seed dreg, a byproduct of oil processing, is rich in many nutrients. However, this is always used as animal fodder and fertilizer. My laboratory has explored the possible use of nutrient-rich peony seed dreg as a functional food. To this end, Peony seed dreg polysaccharides (PSDP) were sequentially extracted using hot buffer (HBSS), chelating agent (CHSS) and dilute alkaline (DASS) followed by concentrated alkaline (CASS). The nutraceutical properties of the various polysaccharides fractions were investigated. The four PSDPs possessed simple composition and showed the characteristic absorptions for polysaccharides at 3600-3200 cm-1 and 3000-2800 cm-1. The functional group of four PSDPs displayed some slight differences. The results of melting point and enthalpy of four samples confirmed a good thermal stability. The emulsifying activity and emulsifying stability of four PSDPs exhibited dose-dependent response, HBSS with the highest emulsifying activity and CHSS with the longest emulsifying stability. Comparison among the antioxidant activities of four PSDPs, CASS showed highest abilities in the DPPH radical scavenging activity, reducing power and ABTS radical scavenging activity, HBSS exhibited the highest hydroxyl radical scavenging activity and CHSS displayed the higher chelating ability on ferrous ions than others. At a liquid-solid ratio less than 2.5% w/w in aqueous solution, the polysaccharides extracted from peony seed presented non-Newtonian shear thinning behavior and could be well described by the Cross model. The peony seed polysaccharides showed predominantly viscous responses (G’

Session Introduction

Siew Young Quek

The University of Auckland, New Zealand

Title: Microencapsulation of bioactives for functional food development

Biography:

Siew Young Quek was graduated with a PhD degree from School of Chemical Engineering, University of Birmingham (UK) in 1999. She is the Director of Food Science Program at University of Auckland. Her current research interests include microencapsulation of bioactives for delivery into functional foods, extraction & characterization of bioactives/functional ingredients from food and waste byproducts, processing & food quality and food product development. She has published more than 200 journals articles and conference proceedings, 4 book chapters and delivered 21 reports for industries. She is a Fellow of the New Zealand Institute of Food Science and Technology.

Abstract:

The increasing pressure on the food industry to produce healthy food products has, as a result, unveiled the challenge of processing functional ingredients in a way that meets the requirements of the modern day consumer. The major issues limiting the use of functional ingredients without prior processing include vulnerability to oxidative breakdown, limited water or lipid solubility, poor thermal stability and poor taste. Unprocessed, these ingredients such as bioactive compounds are essentially unsuitable for use as food ingredients due to any of the aforementioned reasons. This leads to the application of microencapsulation technology as one of the methods in production of food ingredients for functional food application. By utilizing microencapsulation technology, it is possible to produce functional ingredients with desirable chemical and physical properties. This paper aims to provide an understanding on the application of microencapsulation processes to produce functional ingredients for foods. Microencapsulation of lipophilic bioactive components (LBCs) and the development in the area will be discussed.

Jatin R Joshi

Founder & President Instavit Ltd, London, UK.

Title: It’s not the innovation, it’s the application: How vitamins saved my life and career

Biography:

Dr Jatin Joshi completed his medical training from University College London, graduating with honours. He then went on to complete his surgical residency in London with specialisation in Plastic and Reconstructive surgery gaining membership of the Royal College of Surgeons. He is the founder and President of Instavit Ltd, a nutraceutical company that utilises oral spray technology as the delivery vehicle for nutritional supplementation. A confirmed ‘vitamin cynic’ Dr Joshi has become a true believer after these products helped restore his life. He has published several papers, authored a surgical textbook and presented internationally many times.

Abstract:

The need for nutritional supplementation is a contentious issue. It is one that has divided both the scientific community and public opinion. With my background as a surgeon I started as a cynic, I would see undigested tablets in the colons of patients and ingredient lists full of indigestible fillers. My opinion was that in a society where a healthy and well-balanced diet was accessible to most, there was no need to consider vitamin supplementation. All this changed after I was diagnosed with Crohn’s Disease in my early twenties. I was a trainee surgeon working over 110 hours a week whilst my condition progressively deteriorated. Following major emergency abdominal surgery, I was left with severe nutritional deficiencies; the need to take vitamin supplements became essential to my well being, but as with most individuals, my compliance rates were appalling. I had to find another way. The journey for me from a surgeon to an innovator was driven out of a genuine necessity, not only to find a way to increase my compliance but also to help others struggling to achieve a balanced diet or those having difficulty with digestive absorption issues. Approaching the problems from a functional and delivery standpoint, I was able to develop a line of supplements that use oral spray technology to improve compliance and dosage control by way of its delivery system, accessibility and ease of use. It was the answer for me but as I discovered it became the answer for thousands of others.

Session Introduction

Jatin R Joshi

Founder & President Instavit Ltd, London, UK.

Title: It's Not The Innovation, It's The Application: How Vitamins Saved My Life And Career

Biography:

Jatin R Joshi has completed his Medical training from University College London, graduating with honors. He did his Surgical Residency in London with specialization in Plastic and Reconstructive surgery gaining Membership of the Royal College of Surgeons. He is the Founder and President of Instavit Ltd, a nutraceutical company that utilizes oral spray technology as the delivery vehicle for nutritional supplementation. He has published several papers, authored surgical textbooks and presented internationally.

Abstract:

The need for nutritional supplementation is a contentious issue. It is one that has divided both the scientific community and public opinion. With my background as a surgeon I started as a cynic, I would see undigested tablets in the colons of patients and ingredient lists full of indigestible fillers. My opinion was that in a society where a healthy and well-balanced diet was accessible to most, there was no need to consider vitamin supplementation. All this changed after I was diagnosed with Crohn’s Disease in my early twenties. I was a trainee surgeon working over 110 hours a week whilst my condition progressively deteriorated. Following major emergency abdominal surgery, I was left with severe nutritional deficiencies; the need to take vitamin supplements became essential to my well being, but as with most individuals, my compliance rates were appalling. I had to find another way. The journey for me from a surgeon to an innovator was driven out of a genuine necessity, not only to find a way to increase my compliance but also to help others struggling to achieve a balanced diet or those having difficulty with digestive absorption issues. Approaching the problems from a functional and delivery standpoint, I was able to develop a line of supplements that use oral spray technology to improve compliance and dosage control by way of its delivery system, accessibility and ease of use. It was the answer for me but as I discovered it became the answer for thousands of others.

Session Introduction

Baodong Zheng

Fujian Agriculture and Forestry University, China

Title: Sensory and nutraceutical properties of Chinese olive pumice based high fiber biscuit

Biography:

Baodong Zheng has completed his PhD from Fujian Agriculture and Forestry University. He is the Dean of College of Food Science, Fujian Agriculture and Forestry University. He has published more than 20 papers in reputed journals and has been serving as the Editorial Board Member of Journal of Toxicology.

Abstract:

Consumer interest in healthy eating is shifting towards the potential health benefits of specific foods and food ingredients. Unconventional ingredients combined with usual bakery flour yields better acceptable, healthy and nutritious bakery products. The present study aims to evaluate the sensory and nutritional properties of Chinese olive pumace based high fiber biscuit. The high fiber biscuit was formulated using Chinese olive pumace powder (15 %), wheat (100 g), suger powder (33 %) and spices after repeated trials and the recipe was standardized. When subjected to sensory evaluation, the newly formulated high fiber biscuit derived a score of 4.5 ± 0.51, out of a maximum score of 5, for its overall acceptability, indicating the biscuit was well accepted. The results of the nutritional quality and cost of the newly formulated high fiber biscuit showed that it contains the 10.20 g fiber, 1454 Kcal calories and 18.30 g fat in 100 g biscuit. Furthermore, the glycemic index (GI) results indicated that GI of olive high fiber biscuits was 63.68 which was significanly lower than the control one with the GI value of 76.66. Besides, the physical and chemical indicators as well as the microbiological indicators were also up to the Chinese national standards (GB/T20980-2007 and GB7100-2003). Hence, the study proved that the newly formulated olive pumace powder based biscuit is well acceptable based on its sensory properties and fiber rich nutritional quality.

Session Introduction

Norihisa Kato

Hiroshima Universitry, Japan

Title: Novel Beneficial Functions Of Vitamin B6

Biography:

Dr Norihisa Kato is from Hiroshima Universitry, Japan

Abstract:

TBD

Session Introduction

Jiankang Liu

Xi'an Jiaotong University, CHINA

Title: Targeting Mitochondria For Preventing And Treating Metabolic Syndrome With Natural Compounds From Food And Nutrition

Biography:

Jiankang Liu has received his BS from Xi’an Jiaotong University and PhD of Medical Science from Okayama University School of Medicine, Japan. He has completed Post doctorate training in Dr. Bruce Ames Laboratory at University of California, Berkeley and worked as a Faculty at University of California at Berkeley, Children Hospital Oakland Research Institute, University of California at Irvine, University of Kentucky College of Medicine and Shanghai Institute for Nutritional Science, Chinese Academy of Sciences. Currently, he is a Professor and the Dean of Xi’an Jiaotong University School of Life Science and Technology and an Adjunct Professor of the School of Pharmacy, University of Southern California. His research interests include molecular and cellular mechanisms of aging, stress and age/stress associated degenerative diseases with a focus on nutritional regulation of mitochondrial metabolism.

Abstract:

Insulin resistance is an important feature of metabolic syndrome such as type-2 diabetes, obesity and cardiovascular disease. The underlying mechanisms of insulin resistance are still unclear. Mitochondrial dysfunction, including disorders of mitochondrial biogenesis and mitophagy and imbalance of redox system has been suggested to be involved in insulin resistance. Increasing evidence suggests that targeting mitochondria to protect mitochondrial function could prevent and ameliorate various diseases associated with mitochondrial dysfunction forming a unique field, i.e., mitochondrial medicine. In this presentation, I have summarized our recent studies with nutrients to target mitochondria by stimulating mitochondrial homeostasis to improve mitochondrial function and regulate redox balance for preventing and ameliorating metabolic syndrome. We have focused on natural compounds from food and nutrition including olive, bitter gourd and pomegranate to regulate mitochondrial biogenesis and degradation in cellular systems and in animal models. The in vitro and in vivo studies on the effects and mechanisms of mitochondrial targeting nutrients or their combinations may help us to understand the importance and mechanisms of mitochondrial remodeling and to develop mitochondria-targeting agents for preventing and treating obesity, diabetes and cardiovascular disease.

Session Introduction

Prachya Kongtawelert

Chiang Mai University, Thailand

Title: Sesamin: From Sesame To The Functional Foods

Biography:

Prachya Kongtawelert has completed his PhD from Sydney University since 1992. He is currently working as Associate Professor in Department of Biochemistry. He is interested in researches concerning about pathogenesis of osteoarthritis, rheumatoid arthritis, including diagnosis and treatment. Further, he has developed the formulation for dietary supplement products based on the active ingredient of sesamin, which is a natural compound from sesame.

Abstract:

Sesame has been used as food over 4,000 years. It contains very high value of nutritional compounds. One of them is sesamin, which is the most containing of lignan in sesame. There are many research papers concerning about its pharmacological activities. This leading to the research, and development, and apply this natural compound, sesamin, for widely uses as functional foods and cosmetics. It has been studied in our laboratorie as anti-cartilage degradation (as anti-osteoarthritis and anti-rheumatoid arthrisits) by inhibition of the Inteleukin-1 Beta, which is the most potent pro-inflammatoray cytokine, throgh NFkB and NAPK sinalling pathway in human chondrocyte culture. Sesamin shows the chodroprotective effects in cartilage explant culture and papain induced cartilage degradation in rat model. We are also studied anti-osteoporosis as enhancing the mineralisation, and stimulating osteoblast functions by up-regulating Type-II Collagen, alkaline phosphatase, and bone morphogenetic protein-2 (BMP-2). The osteoclastogenesis was inhibited by sesamin. Furthermore, the neuro-protective effect on the LPS-induced neuronal cell (PC12) development has been studied. Finally, the pathway of commercialization for development of simple and innovative dietary supplement of healthy products from sesamin with other natural resources, such as black rice bran and pre-cooked rice have been succesfully formulated and sold in several countries, especially in Southeast Asian.

Session Introduction

Guangjian Jiang

Beijing University of Chinese Medicine, China

Title: Ginsenoside Rb1 Promotes Browning Through Regulation Of PPARγin 3T3-L1 Adipocytes

Biography:

Guangjian Jiang has received her BS from North China Coal Medical University and PhD in Medical Science from Hebei Medical University. She has completed Post doctorate training in State Key Laboratory of Reproductive Biology, The Institute of Zoology of Chinese Academy of Sciences and worked as a Post Doctor at Harvard University Medical School Beth Israel Deaconess Medical Center in Boston. She has worked as a Dean of Department of Biochemistry and Molecular Biology in North China University of Science and Technology. Currently, she is a Professor in Diabetes Research Center, Beijing University of Chinese Medicine. Her research interests include natural medicine (traditional Chinese medicine) treatment of chronic disease and majored in molecular and cellular mechanisms of diabetes, cancer and age-associated degenerative diseases. She has focused on the Epigenetics, Traditional Chinese Medicine and Reproductive Biology areas.

Abstract:

Browning of white adipocyte tissue (WAT) has received considerable attention due to its potential implication in preventing obesity and related comorbidities. Ginsenoside Rb1 is reported to improve glycolipid metabolism and reduce body weight in obese animals. However whether the body reducing effect mediates by browning effect remains unclear. For this purpose, 3T3-L1 adipocytes were used to study the effect of ginsenoside Rb1 on browning adipocytes specific genes and oxygen consumptions. The results demonstrate that 10 mM of ginsenoside Rb1 increases basal glucose uptake and promoted browning evidenced by significant increases in mRNA expressions of UCP-1, PGC-1a and PRDM16 in 3T3-L1 mature adipocytes. Further, ginsenoside Rb1 also increases PPARγ activity. And the browning effect is abrogated by GW9692, a PPARγ antagonist. In addition, ginsenoside Rb1 increases basal respiration rate, ATP production and uncoupling capacity in 3T3-L1 adipocytes. Those effects are also blunted by GW9692. The results suggest that ginsenoside Rb1 promote browning of 3T3-L1 adipocytes through induction of PPARγ. Our finding offer a new source to discover browning agonists and also useful to understand and extend the applications of ginseng and its constituents.

Session Introduction

Siew Young Quek

University of Auckland, New Zealand

Title: Microencapsulation Of Bioactives For Functional Food Development

Biography:

Assoc. Professor Siew-Young Quek graduated with a PhD degree from School of Chemical Engineering, University of Birmingham (UK) in 1999. She is the Director of Food Science Programme at University of Auckland. Her current research interests include microencapsulation of bioactives for delivery into functional foods, extraction & characterisation of bioactives/ functional ingredients from food and waste by-products, processing & food quality and food product development. She has published more than 200 journals articles and conference proceedings, 4 book chapters and delivered 21 reports for industries. She is a Fellow of the New Zealand Institute of Food Science and Technology

Abstract:

The increasing pressure on the food industry to produce healthy food products has, as a result, unveiled the challenge of processing functional ingredients in a way that meets the requirements of the modern day consumer. The major issues limiting the use of functional ingredients without prior processing include vulnerability to oxidative breakdown, limited water or lipid solubility, poor thermal stability, and poor taste. Unprocessed, these ingredients such as bioactive compounds are essentially unsuitable for use as food ingredients due to any of the aforementioned reasons. This leads to the application of microencapsulation technology as one of the methods in production of food ingredients for functional food application. By utilising microencapsulation technology, it is possible to produce functional ingredients with desirable chemical and physical properties. This paper aims to provide an understanding on the application of microencapsulation processes to produce functional ingredients for foods. Microencapsulation of lipophilic bioactive components (LBCs) and the development in the area will be discussed.

Session Introduction

Toshikazu Suzuki

Wayo Women’s University, Japan

Title: Changes In Blood Coenzyme Q10 Levels By Food Intervention And Intake Of CoQ10-fortified Rice

Biography:

Toshikazu Suzuki has completed his PhD at the age of 27 years from Graduate School of Pharmaceutical Sciences, Chiba University and postdoctoral studies from National Institute of Infectious Disease, Japan. He is the Associate Professor of Department of Health and Nutrition, Wayo Women’s University. He has published more than 60 papers in Journals and has been serving as a councilor of The Japanese Clinical Nutrition Association.

Abstract:

non-essential nutrient, coenzyme Q10 (CoQ10) is a key element in mitochondrial energy production and antioxidant protection. Daily intake of CoQ10 is not considered in nutritional guidance or menu planning. Hospitalized older people have lower blood levels of CoQ10 with possible decreased intake of CoQ10 compared with healthy older people, suggesting that adequate intake of CoQ10 maintains wellness in older people. First we estimated daily intake of CoQ10 from food, designed a food intake guide for ingestion of increased amounts of CoQ10 with balanced food choice and evaluated the usability in a diet intervention trial. Average daily intake of CoQ10 from food was 1.9 mg/1000 kcal/day in both men and women. Ratio of dietary animal to vegetable protein was involved in the amount of CoQ10 intake. Our food intervention was effective in increasing CoQ10 intake at up to 1mg/day while maintaining PFC balance. However, choice of food items was sometimes a burden to the participants. Next, we investigated the effect of food choice and efficacy of CoQ10-fortified food on blood CoQ10 levels. Two weeks prohibition of meat/poultry consumption decreased blood CoQ10 levels by ~0.1µg/mL. Eating 300 g/day of CoQ10-fortified boiled rice (13 mg CoQ10/100g rice) could increase both intake and blood levels of CoQ10. Our results indicate that the choice of a CoQ10-fortified food may be more applicative for keeping/raising blood CoQ10 levels than food intervention. Further study of the effect of CoQ10-fortified food intake on maintaining/improving the quality of life of the older people should be pursued.