Nutraceuticals and Nutrition Supplements

Biography

Biography: Natanamurugaraj Govindan

Abstract

Commelina nudiflora L. aqueous extract as a reducing and stabilizing agent for the synthesis of metallic gold and silver nanoparticles in green chemistry approaches. The biosynthesized gold and silver nanoparticles have studied their physicochemical and biological properties in nanoscale regime. The synthesized gold and silver nanoparticles physicochemical properties were characterized by various analytical techniques such as UV-VIS, FESEM, XRD and FT-IR. The outcome shows that the physicochemical characterizations of gold and silver nanoparticles were crystalline in nature with size range between 25-45 nm and 50-150 nm respectively. The EDX spectrum was shown strong signal energy peaks of both gold and silver atoms in 2-3 keV. The in vitro antioxidant ability of C. nudiflora extracts were studied using DPPH and ABTS radical scavenging assay. Moreover the C. nudiflora plant extract synthesized gold and silver metal nanoparticles have significantly control the proliferation of HCT-116 colon cancer cells in in vitro. The biosynthesized gold and silver nanoparticles were showed reduced cell viability and increased cytotoxicity on HCT-116 colon cancer cells with IC50 concentration of 200 and 100 µg/ml. Furthermore, the flow cytometry experiments showed that the IC50 concentrations of gold and silver nanoparticles treated cells are increased DNA fragmentation and significant changes were observed in sub G1, S and G2 cell cycle phases compared with positive control. Additionally, the mRNA gene expressions of HCT-116 cells were studied by RT-qPCR techniques. The pro-apoptotic genes are highly expressed in the gold nanoparticles treated HCT-116 colon cancer model. However, the C. nudiflora extract as a novel source for synthesis of metallic gold and silver nanoparticles with controlled size and shapes and also it could be potent anti-colon cancer drug in the near future.