Day 2 :
- Nutraceuticals in Prevention of Disease
Nutraceuticals Used to Support the Healing Process in Chronic Disease
Herbals and Natural health products
Recent Trends in Nutraceuticals
UNESCO Chair- Life Sciences International Postgraduate Educational Center, Armenia
Prof. Sinerik Ayrapetyan has completed his Ph.D in Cell Biophysics in the Bogomolets Institute of Physiology (NAS of Ukraine). He is the coordinator of UNESCO Chair and head of Research Council of Life Sciences International Postgraduate Educational Center. His current research interest is “Intracellular Signaling System in Norm and Pathology”. On this subject he has published 7 international books and 115 papers. He is a member of the board of associate editors for the journals: “Electromagnetic Biology and Medicine”, “ISRN Biophysics” and a member of editorial board for the journals: “Advances in Life Sciences”, “Bioavailability and Bioequivalence”, “Applied Pharmacy”, “European Journal of Biophysics”, “Biochimica et Biophysica Acta” , “Journal of International Dental and Medical Research”. He is a member of International Coordination Council of WHO and number of professional international societies.
Age-induced medical disorders are modern problems in medicine and have become a focal point for public health concern due to the aging of the world population. The main barrier for solving this problem is, in the literature available for us, the absence of a reliable, universal biomarker for detection of the functional state of the whole organism though there are different methods for detection of the functional state of individual organs. We believe that metabolic controlling of cell hydration could serve as a universal biomarker for estimation of the effect of different chemical and physical factors, including food and drinking water, on the functional state of the organism. By our previous study it was shown that cell pathology, including aging, leads to the dysfunction of Na+/K+-ATPase α3 isoform-dependent intracellular signaling system controlling cell hydration. Therefore, we suggest that that activation of α3 isoform-dependent cell hydration could serve as a marker for estimation of bioavailability of food and drinking water. For this purpose elaboration of a new non-invasive device with corresponding software for detection of muscle hydration based on frequency-dependent characteristics of muscle impedance is suggested. The experimental data that prove our suggestion will be demonstrated in the lecture.
Wrocław University of Environmental and Life Sciences, Poland
Title: Formulation of blackcurrant powders – The effect of the drying method on selected quality parameters
Time : 10:55-11:30
Anna Michalska has completed her PhD at the age of 29 years from Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences in Olsztyn (Poland) and postdoctoral studies from HESSO Valais-Wallis University of Applied Sciences and Art in Sion (Switzerland). Meanwhile, she was attended a scholarship ‘TOP500 Innovators – science, management, commercialization’ at Stanford University (CA, USA). Currently, she is working at Wroclaw University of Environmental and Life Sciences as an assistant professor. She has published more than 20 papers in journals with IF and has been serving as an associate editor of International Journal of Food Engineering (De Gruyter).
Currently, powdered form of fruits has received a special attention as reducing water content in powders increases shelf life and extends the availability of fruits throughout the year, being a valuable source of bioactive compounds, including antioxidants. Processing involved in the production of blackcurrant powders is a multi-step and time-consuming procedure that may influence the functional properties of the products obtained. In the present study, the possible ways of formulation of blackcurrant powders were presented. A comparison of blackcurrant powders gained by selected drying methods was shown in relation to changes in the content of nutritional bioactive compounds and antioxidant capacity against the quality of dehydrated products. The application of high-temperature drying methods was performed in comparison to the freeze-drying process. The properties of powders were characterized by physical parameters providing data on their handling and storage properties. The data obtained showed the ways of retention of major bioactive compounds by modulating the processes applied for blackcurrant powders production that may be used as nutraceuticals with a broad range of applications.
Reckitt Benckiser, USA
Time : 11:30-12:05
Nalin Siriwardhana is a Senior Medical Scientist with proven track records of both academic and industrial careers. He has expertise in applications of nutraceuticals for a wide array of health conditions including heart, digestive, bone, joint, immune, diabetes, cancer, cognition, eye and sleep complications. Followed by a successful academic career as a Research Assistant Professor at the University of Tennessee and Texas Tech University, he has joined RB as a Senior Associate-Global Medical & Innovation - VMS. He has published more than 20 scientific articles. He has presented more than 30 times at Nutrition Science Conferences. In his current role at RB, he contributes his medical and nutrition science expertize for product innovations, global launches, medical claims and medical detailing. Also, he is the founder of HONcode certified website ‘www.nutritionremarks.com’ which publishes health and nutrition news based on scientific evidence.
Omega-3 fatty acids are essential nutrients for the human body. Despite a few recent controversial scientific reviews and meta-analysis regarding the benefits of omega-3s, the global omega-3 market has been projected to reach upto $34B in 2016. Furthermore, there is continuous and growing interest towards further exploring the science behind omega-3s. The number of scientific studies appearing in PubMed has exceeded 21,000 publications. Also, there is a critical knowledge gap of properly designed clinical studies linking individual variability in utilization of omega-3s with long term benefits. This presentation discusses the major mechanisms by which omega-3 delivers health benefits, both positive and negative consistent outcomes from clinical studies, and scientific facts contributed to why omega-3s have been regarded amongst the top nutrients of all time. In modern western diet, the ratio between omega-3 and omega-6 is over 1:20, whereas human beings evolved on a diet with a ratio of approximately 1:1 or 1:2. This change has occurred within an extremely short time period as compared to the evolutionary time scale of the human genome. Given the well-established importance of gene-nutrient compatibility, the dramatic shift of the omega-3 to omega-6 ratio may be the underlying reason for many chronic diseases. Therefore, intake of nutrients compatible with our genome may ensure proper and efficient functioning of the human body, thus contributes towards the prevention of a variety of diseases.
Amway Corporation, USA
Title: Ingredient innovation in weight management: New approaches for the development of functional botanical ingredients for innovative products
Time : 12:05-12:40
Jat Rana earned his Masters of Science in Applied Organic Chemistry and PhD in Organic Chemistry from Birmingham University, England (UK). He has done his Post-doctoral fellow at the University of Glasgow, Scotland (1981) and USDA Research fellow at University of Minnesota, Minneapolis (1985). Production Manager at the Sigma-Aldrich Corporation in St. Louis, MO (1985-1996). In 1996 he joined the Nutraceutical R&D group at Amway Corporation in Ada, Michigan, where he continues to provide his expertise in Natural products / Herbal chemistry and Asian traditional medicine. Discovery of innovative ingredients from plants for health & beauty application is his passion. He has published many scientific papers in peer reviewed journals.
Use of botanicals for improving or to care for human health has evolved independently in different countries worldwide. In the scientific area, botanical products are generally intended as drugs, medicinal products, food supplements, or substances for therapeutic use derived from raw material of whole plants or parts of them. With the recent advent of high-throughput screening technologies, experimental analyses of the active ingredients and their mechanisms of action have become increasingly important along with the standardization of botanicals. After thousands of years of clinical practice and high-throughput screening & evaluation, scientists have discovered a variety of botanical ingredients possessing medicinal value. With accumulated experience and knowledge, the relationship between efficacy of ingredient and certain diseases or symptoms was gradually established. This presentation will focus on examples of various biological screening & extraction technology platform implemented for the discovery of innovative science based functional botanical ingredients for the development of Weight Management. This presentation will be of great interest to various R&D scientists looking for the innovation in ingredient technology and consumers who use new and innovative products.
University of Balamand, Lebanon
Time : 12:40-13:15
In 2011, in recognition of her accomplishments, Dr. Abdel-Massih was named by The World Academy of Sciences (TWAS) a “TWAS Young Affiliate” for a period of five years. Based in Trieste, TWAS works to advance scientific work that contributes toward sustainable prosperity in the developing world. The author of 19 publications in peer reviewed journals; Dr. Abdel-Massih is currently studying the biological activity of plant products, looking for anti-bacterial molecules within plant extracts. Her focus is on the biotransformation of molecules and the effect of Lebanese plant extracts on multi-drug
Pectin is a heterogeneous polysaccharide mainly present in citrus fruits and has different biological activities. Its chemical composition and structure varies between different plant sources and even in different parts of a plant. In this study, the cytotoxicity, anti-proliferative, and antimicrobial activity of citrus pectin (high molecular weight pectin) and modified citrus pectin (MCP) were investigated. Cytotoxicity of various concentrations of pectin and MCP was studied against HaCaT cell line (human keratinocyte cell line) using Trypan blue method and LDH-cytotoxicity assay. Anti-proliferative activity was assayed using a WST-1 proliferation kit. MCP and Pectin both reduced the viability of HaCat cells in a dose dependent manner; however MCP was found to be more cytotoxic than high molecular weight citrus pectin since it had a lower IC50 (300ug/ul).MCP was also more capable of inhibiting HaCat proliferation than pectin. The antibacterial activity of citrus pectin and MCP was investigated against reference strains and clinical isolates of Staphylococcus aureus and extended spectrum beta-lactamase (ESBL) producing Escherichia coli. Broth micro dilution method was used to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The antibacterial activity of pectin varied according to the source of the pectin extract, the pH used, and the molecular weight of pectin. The greatest antibacterial activity was observed with pectin at pH 6. The MIC values against S. aureus ranged between 0.39–3.125 mg/ml and the MBC values ranged between 3.125– 12.5mg/ml. Modified Citrus Pectin (MCP) exhibited lower antibacterial activity with MICs of 25-50 mg/ml and MBCs were equal or higher than 50 mg/ml. Pectin exhibited lower antibacterial activity against E. coli with MICs of 25 and 50 mg/ml and MBCs ranging between 25 and 50 mg/ml. The conclusions from this study suggest that MCP exhibit a higher anti-proliferative effect on HaCaT cell line whereas pectin showed a stronger antibacterial activity against S. aureus, a major Gram positive pathogen. The most probable explanation of this observation is the different effect due to the variable length, molecular weight, and exposed side-chains of MCP and high molecular weight citrus pectin. For that they merit further investigation as a potential therapeutic agent.
University of Hawaii, India
Time : 14:00-14:35
Dr. Parimelazhagan Thangaraj, is Associate Professor in the Department of Botany, School of Life Sciences, Bharathiar University, Coimbatore. His areas of specialization include Bioprospecting of medicinal plants. He has worked as Scientist in DRDO, Govt. of India. He has filed four patents and has more than 35 research publications to his credit. He is working on natural products for the past 10 years and guiding 8 Ph.D. scholars. His book ‘Herbal Perspective: Present and Future’ is an excellent reference book for those biologists and pharmacy students. He has four books to his credit on various aspects of medicinal plants and filed 4 patents. He has bagged National Science Day Medal 2005 from DRDO HQ, New Delhi and Laboratory Scientist of the Year award 2005 from DRDO.
Syzygium calophyllifolium belongs to the family Myrtaceae. It is endemic to the medicinal wealth of Western Ghats of India and used as wild edible, rich in essential nutrients fruit by tribal peoples due to its traditional medicinal properties. S. calophyllifolium fruit and bark is placed in the aching tooth and inflammation for relief. Seed extract has shown profound antidiabetic effect. The present investigation was carried out to evaluate the antioxidant activity and nutritional content of S. calophyllifolium fruit. The extraction was carried out in Soxhlet apparatus using petroleum ether, ethyl acetate, methanol and, finally, with hot water maceration. Methanol extract of fruit showed good nitric oxide radical scavenging (65.6%) and metal chelating activity (44.1 mg EDTA equivalents/ g extract). The analysis of amino acid was done using amino acid analyzer (HPLC- LC 10 AS). It was found that almost all essential and non-essential amino acids were present with arginine (8.4 % of protein) and cysteine (7.4 % of protein) in highest amounts. The results were compared with WHO/FAO recommended levels of amino acids. The mineral estimation showed the presence of calcium (12200 part per million) nitrogen (8800 part per million) in superior quantity when compared to other minerals. High amount of proteins (20.45 g/100g) and carbohydrates (41.35 g/100g) were also estimated in this fruit. This is the first report on the nutritional composition of S. Calophyllifolium which will enhance its importance as a source of medicinal food, and help in the exploration of nutraceutical properties of the fruit with its wide commercialization in Indian as well as International market.
Nitika has completed her Graduation in Pharmacy in 2015 from the Khalsa College of Pharmacy, Punjab, India. During her Graduation, she has been successful in publishing one review article in a reputed journal and also got an opportunity to attend an International Conference on Advances in Pharmaceutical Nanotechnology and Nanomedicine (ICPNN-2015).
Nutraceuticals is combination of two words ‘nutrition’ and ‘pharmaceuticals’, invented by Stephen L. DeFelice in 1989. Nutraceuticals can be considered as food or as a part of food which plays a vital role in maintaining the health status, regulating immunity and thus treating as well as curing of a various diseases like cardiovascular disease, neurological disease, diabetes etc. By increasing awareness levels about health and fitness, urged by media coverage are prompting the majority of masses to lead healthier lifestyles. In a growing percentage of vascular disease etiologies, atherosclerosis is one of the most common ailments observed. Polyunsaturated Fatty Acids are found to reduce the formation of thromboxane and hence, are useful as preventive measures for atherosclerosis. Omega-3-fatty acids have been found to be useful for the reduction of LDL and VLDL levels and increase in physicochemical stability of cell wall. Besides, nutraceuticals exhibit an anti-inflammatory and anti-oxidative properties as well which provide useful measures for preventing and treating diabetic complications. There are a wide variety of neuro-degenerative diseases with different symptoms and pathologies which can be cured by nutritional supplements. Oxidative stress plays a fundamental role in aging and neurodegeneration. In recent studies, it has been proved that blueberries have found with highest antioxidant content which act by normalize sensitivity to neurotransmitters and promote synaptic plasticity.